IADR Abstract Archives
Resveratrol’s Effect on Biofilm Formation of Streptococcus mutans
Objectives: Resveratrol is an antioxidant found in grapes, wine, blueberries, peanuts, cocoa and many other plant sources. Resveratrol is made in these plants to fight fungal infection, ultraviolet radiation, stress, and injury. Streptococcus mutans is the main bacterium that causes tooth decay leading to dental caries. Previous research has shown that nicotine increases biofilm formation of S. mutans. The objective of this study was to identify how Resveratrol affects biofilm formation of S. mutans with and without the presence of nicotine.
Methods: To start, S. mutans strain UA159 was incubated in a microtiter plate for 24 hours with dilutions of resveratrol (0, .98, 1.95, 3.9, 7.8, 15.6, 31.25, 62.5, 125, 250, and 500 ug/mL) in Tryptic Soy Broth with 1% sucrose (TSBS). Biofilm formation was measured using a crystal violet staining assay. For the next experiment, S. mutans strain UA159 was incubated in a microtiter plate for 24 hours with dilutions of nicotine (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) in TSBS, with and without 62.5 ug/mL resveratrol.
Results: The results demonstrated significant inhibition of S. mutans biofilm formation occurred at 31.25 ug/mL resveratrol. Biofilm formation was significantly inhibited by 62.5 ug/mL resveratrol at all concentrations of nicotine.
Conclusions: The results of this study demonstrate that eating foods or drinking beverages containing resveratrol may have health benefits of reducing S. mutans biofilm formation in the oral cavity leading to less dental caries, even with patients who smoke. Resveratrol shows inhibitory effects to S. mutans with and without the presence of nicotine.
Methods: To start, S. mutans strain UA159 was incubated in a microtiter plate for 24 hours with dilutions of resveratrol (0, .98, 1.95, 3.9, 7.8, 15.6, 31.25, 62.5, 125, 250, and 500 ug/mL) in Tryptic Soy Broth with 1% sucrose (TSBS). Biofilm formation was measured using a crystal violet staining assay. For the next experiment, S. mutans strain UA159 was incubated in a microtiter plate for 24 hours with dilutions of nicotine (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) in TSBS, with and without 62.5 ug/mL resveratrol.
Results: The results demonstrated significant inhibition of S. mutans biofilm formation occurred at 31.25 ug/mL resveratrol. Biofilm formation was significantly inhibited by 62.5 ug/mL resveratrol at all concentrations of nicotine.
Conclusions: The results of this study demonstrate that eating foods or drinking beverages containing resveratrol may have health benefits of reducing S. mutans biofilm formation in the oral cavity leading to less dental caries, even with patients who smoke. Resveratrol shows inhibitory effects to S. mutans with and without the presence of nicotine.