IADR Abstract Archives
STRO-1-sorted Human and Porcine Tooth-Germ Stem Cells for Osteogenic Differentiation
Objectives: Objective of this study is to compare osteogenic differentiation potential of STRO-1-positive selected stem cells from human (hTGSCs) and porcine (pTGSCs) tooth germs and establish whether domestic pig is a useful model for cell-mediated bone-tissue engineering.
Methods: hTGSCs and pTGSCs were isolated from mandibular third molar tooth germs of humans and domestic pig, characterized by expression of CD105, CD90, CD44, CD45, CD34, sorted according to STRO-1 expression as STRO-1(+) and STRO-1(-). STRO-1(+), STRO-1(-) and unsorted-heterogenous cells (US) were tested for differentiation towards osteoblast-like phenotype in osteogenic medium. Groups were compared for proliferation, ALP activity, calcium deposition throughout 21 days of incubation and statistically analyzed by SPSS (p<0.05).
Results: hTGSCs and pTGSCs both showed positivity for CD44, CD90, CD105; negativity for CD45 and CD34. STRO-1(+)-sorted cell fraction from heterogenous population of pTGSCs (27.4%) was higher than that of hTGSCs (11%). Both cell types showed significant increase in proliferation up to 14 days in all groups, followed by decrease in cell number in STRO-1(+)/OM, STRO-1(-)/GM, STRO-1(-)/OM of pTGSCs; STRO-1(+)/OM and US/OM of hTGSCs due to limited area available for cell growth. STRO-1(+)-pTGSCs showed highest proliferation rates throughout culture time whereas STRO-1(+)-hTGSC-groups did not. Addition of osteogenic-medium suppressed proliferation in US groups of both cell types. All groups of both cell types synthesized high amounts of ALP being the highest in US/OM groups on days 4, 14. Calcium deposition increased in all cell groups incubated in osteogenic media. There was no significant difference between hTGSC groups in calcium content, however STRO-1(+)-pTGSCs accumulated significantly more calcium after 21 days of culture.
Conclusions: Both cell types showed similar osteogenic behavior and STRO-1 selection proved to be not advantageous in enhancement of osteogenic differentiation since TGSCs might maintain their role efficiently with cell-to-cell interactions in a heterogenous population. Thus, domestic pig was thought to be a useful model for dental stem cell-mediated bone tissue engineering since results were similar for hTGSCs and pTGSCs.
Methods: hTGSCs and pTGSCs were isolated from mandibular third molar tooth germs of humans and domestic pig, characterized by expression of CD105, CD90, CD44, CD45, CD34, sorted according to STRO-1 expression as STRO-1(+) and STRO-1(-). STRO-1(+), STRO-1(-) and unsorted-heterogenous cells (US) were tested for differentiation towards osteoblast-like phenotype in osteogenic medium. Groups were compared for proliferation, ALP activity, calcium deposition throughout 21 days of incubation and statistically analyzed by SPSS (p<0.05).
Results: hTGSCs and pTGSCs both showed positivity for CD44, CD90, CD105; negativity for CD45 and CD34. STRO-1(+)-sorted cell fraction from heterogenous population of pTGSCs (27.4%) was higher than that of hTGSCs (11%). Both cell types showed significant increase in proliferation up to 14 days in all groups, followed by decrease in cell number in STRO-1(+)/OM, STRO-1(-)/GM, STRO-1(-)/OM of pTGSCs; STRO-1(+)/OM and US/OM of hTGSCs due to limited area available for cell growth. STRO-1(+)-pTGSCs showed highest proliferation rates throughout culture time whereas STRO-1(+)-hTGSC-groups did not. Addition of osteogenic-medium suppressed proliferation in US groups of both cell types. All groups of both cell types synthesized high amounts of ALP being the highest in US/OM groups on days 4, 14. Calcium deposition increased in all cell groups incubated in osteogenic media. There was no significant difference between hTGSC groups in calcium content, however STRO-1(+)-pTGSCs accumulated significantly more calcium after 21 days of culture.
Conclusions: Both cell types showed similar osteogenic behavior and STRO-1 selection proved to be not advantageous in enhancement of osteogenic differentiation since TGSCs might maintain their role efficiently with cell-to-cell interactions in a heterogenous population. Thus, domestic pig was thought to be a useful model for dental stem cell-mediated bone tissue engineering since results were similar for hTGSCs and pTGSCs.