IADR Abstract Archives
Comparative Effects of FGF and PGE2 on OTM and OIIRR
Objectives: To compare the role of an osteogenic cell stimulant i.e. Prostaglandin E2 (PGE2) and a cytokine that is stimulant of progenitors for osteoblast differentiation and osteoclasts i.e. basic Fibroblast Growth Factor (bFGF) on orthodontic tooth movement (OTM) and Orthodontically Induced Inflammatory Root Resorption(OIIRR).
Methods: The study sample consisted of sixty, 8-week-old male Wistar albino rats, weighing 200–230 grams that were randomly divided into five equal groups. Control group received 20 µL of PBS and orthodontic appliance for protraction of first molar was installed. Experimental groups of FGF10, FGF100, FGF1000, received 10, 100, and 1000 ng of bFGF respectively and PGE2 group received 50 µg of prostaglandin E2 and closed coil spring (NiTi) as orthodontic appliance was installed for all groups. After 21 days, the rats were sacrificed, the upper jaws were dissected and the distance between the first and second right molars was measured. Under light microscope, root resorptive lacunae and number of blood vessels were calculated by computer software. Kruskal-Wallis statistical test was used for purpose of the comparison.
Results: Orthodontic tooth movement (OTM) (in millimeter) in Control, FGF10, FGF100, FGF1000, and PGE2 were 0.25±0.13, 0.53±0.11, 0.66±0.20, 0.77±0.23, and 0.48±0.18 respectively and area of resorptive lacunae (square millimeter) were [1.89±0.18, 2.31±0.16, 1.96±0.01, 1.27±0.01, 1.92±0.21] x10E-2 respectively. OTM was significantly higher in experimental groups (p<0.05) and was maximum in FGF1000. OIIRR was minimum in FGF1000 and number of blood vessels, osteoclasts and Howship’s lacunae were significantly higher in E1000 compared to Control group(P<0.05). Maximum amount of root resorption was shown in FGF10 group.
Conclusions: FGF has a dose-dependent effect on the rate of OTM and OIIRR. PGE2 shows acceleration of OTM comparable to FGF10 but OIIRR is greater in FGF10. OTM and OIIRR are complicated phenomena and interpreting the precise molecular events contributing in tooth movement requires more investigations.
Methods: The study sample consisted of sixty, 8-week-old male Wistar albino rats, weighing 200–230 grams that were randomly divided into five equal groups. Control group received 20 µL of PBS and orthodontic appliance for protraction of first molar was installed. Experimental groups of FGF10, FGF100, FGF1000, received 10, 100, and 1000 ng of bFGF respectively and PGE2 group received 50 µg of prostaglandin E2 and closed coil spring (NiTi) as orthodontic appliance was installed for all groups. After 21 days, the rats were sacrificed, the upper jaws were dissected and the distance between the first and second right molars was measured. Under light microscope, root resorptive lacunae and number of blood vessels were calculated by computer software. Kruskal-Wallis statistical test was used for purpose of the comparison.
Results: Orthodontic tooth movement (OTM) (in millimeter) in Control, FGF10, FGF100, FGF1000, and PGE2 were 0.25±0.13, 0.53±0.11, 0.66±0.20, 0.77±0.23, and 0.48±0.18 respectively and area of resorptive lacunae (square millimeter) were [1.89±0.18, 2.31±0.16, 1.96±0.01, 1.27±0.01, 1.92±0.21] x10E-2 respectively. OTM was significantly higher in experimental groups (p<0.05) and was maximum in FGF1000. OIIRR was minimum in FGF1000 and number of blood vessels, osteoclasts and Howship’s lacunae were significantly higher in E1000 compared to Control group(P<0.05). Maximum amount of root resorption was shown in FGF10 group.
Conclusions: FGF has a dose-dependent effect on the rate of OTM and OIIRR. PGE2 shows acceleration of OTM comparable to FGF10 but OIIRR is greater in FGF10. OTM and OIIRR are complicated phenomena and interpreting the precise molecular events contributing in tooth movement requires more investigations.