IADR Abstract Archives
Directing Human iPS Cells Toward PDL Stem Cells
Objectives: Periodontal ligament (PDL) tissue plays an important role in the maintenance of teeth. Stem cells contained in the PDL tissue are known to have the potential to repair and regenerate peripheral bone, cementum and PDL fiber. However, the PDL stem cells (PDLSCs) are difficult to obtain, due to their limited cell number in PDL tissue. Therefore, we focused on the use of induced pluripotent stem (iPS) cells. In this study, we aimed at establishing PDLSCs from the iPS cells.
Methods: Human iPS cells developed from skin fibroblasts were purchased from RIKEN (HPS No.0063). First, iPS cells were differentiated into neural crest-like cells. Then, p75NTR positive cells were isolated from these cells by magnetic activated cell sorting (designated as iPS-NC cells). Moreover, they were cultured on the extracellular matrix produced by human PDL cells (designated as iPS-NC-PDL cells). iPS-NC-PDL cells were characterized as follows, (1) the gene expression of ES and NC cell markers, (2) the gene expression of PDL-related markers, (3) the expression of mesenchymal stem cell (MSC) markers, and (4) the multipotency of iPS-NC-PDL cells.
Results: (1) iPS-NC-PDL cells decreased in the gene expression of ES and NC cell markers compared with iPS and iPS-NC cells. (2) iPS-NC-PDL cells increased the gene expression of PDL-related markers, compared with iPS-NC cells cultured on the fibronectin- and laminin-coated dish. (3) iPS-NC-PDL cells were rich in MSC markers. (4) iPS-NC-PDL cells cultured in osteoblastic or adipocytic differentiation medium differentiated into osteoblast-like cells and adipocyte-like cells, respectively.
Conclusions: iPS-NC-PDL cells decreased in the gene expression of ES and NC cell markers, whereas these cells increased in the gene expression of PDL-related markers and were rich in MSC markers. Thus, iPS-NC-PDL cells might be PDLSC-like cells with the multipotency.
Methods: Human iPS cells developed from skin fibroblasts were purchased from RIKEN (HPS No.0063). First, iPS cells were differentiated into neural crest-like cells. Then, p75NTR positive cells were isolated from these cells by magnetic activated cell sorting (designated as iPS-NC cells). Moreover, they were cultured on the extracellular matrix produced by human PDL cells (designated as iPS-NC-PDL cells). iPS-NC-PDL cells were characterized as follows, (1) the gene expression of ES and NC cell markers, (2) the gene expression of PDL-related markers, (3) the expression of mesenchymal stem cell (MSC) markers, and (4) the multipotency of iPS-NC-PDL cells.
Results: (1) iPS-NC-PDL cells decreased in the gene expression of ES and NC cell markers compared with iPS and iPS-NC cells. (2) iPS-NC-PDL cells increased the gene expression of PDL-related markers, compared with iPS-NC cells cultured on the fibronectin- and laminin-coated dish. (3) iPS-NC-PDL cells were rich in MSC markers. (4) iPS-NC-PDL cells cultured in osteoblastic or adipocytic differentiation medium differentiated into osteoblast-like cells and adipocyte-like cells, respectively.
Conclusions: iPS-NC-PDL cells decreased in the gene expression of ES and NC cell markers, whereas these cells increased in the gene expression of PDL-related markers and were rich in MSC markers. Thus, iPS-NC-PDL cells might be PDLSC-like cells with the multipotency.