IADR Abstract Archives
Use of Glucose in Glycogen Metabolism in Iodine Non-stained (Oral Dysplastic/Malignant) Epithelium
Objectives: Vital staining with iodine solution has been used to distinguish dysplastic/malignant oral epithelium from normal mucosa. It is thought that iodine reacts with glycogen. Oral squamous cell carcinoma (OSCC) cells lacks glycogen and showed no-stain reaction with iodine. Therefore, the purpose of this study is to analyze use of glucose in glycogen metabolism in iodine non-stained (oral dysplastic/malignant) epithelium.
Methods: Twelve frozen tissue samples of iodine-stained and -unstained mucosa were obtained from 12 cases of OSCC. Serial frozen sections were cut and examined with hematoxylin-eosin (HE), periodic acid Schiff (PAS) methods and immunohistochemical staining for the enzyme glycogen synthesis (Glucokinase, GK and Phosphoglucomtase3, PGM3) and the enzyme glycogen phosphorylase (Glucose-6-phosphatase, G6P).
Results: There was no significant difference in immunoreaction for PGM3 between iodine stained and non-stained epithelium. While, iodine non-stained epithelium presented significantly higher immunoreactions for GK and G6P in basal and parabasal layers compared to iodine stained epithelium.
Conclusions: The results of this study suggested that dysplastic/malignant oral epithelium had higher activation in utilization of glucose (higher activation of glucokinase and glucose-6-phosphatase), which results in an activation of glycolysis, while no activation of glucose metabolism to glycogen.
Methods: Twelve frozen tissue samples of iodine-stained and -unstained mucosa were obtained from 12 cases of OSCC. Serial frozen sections were cut and examined with hematoxylin-eosin (HE), periodic acid Schiff (PAS) methods and immunohistochemical staining for the enzyme glycogen synthesis (Glucokinase, GK and Phosphoglucomtase3, PGM3) and the enzyme glycogen phosphorylase (Glucose-6-phosphatase, G6P).
Results: There was no significant difference in immunoreaction for PGM3 between iodine stained and non-stained epithelium. While, iodine non-stained epithelium presented significantly higher immunoreactions for GK and G6P in basal and parabasal layers compared to iodine stained epithelium.
Conclusions: The results of this study suggested that dysplastic/malignant oral epithelium had higher activation in utilization of glucose (higher activation of glucokinase and glucose-6-phosphatase), which results in an activation of glycolysis, while no activation of glucose metabolism to glycogen.