IADR Abstract Archives
Effects of Allantoin on Periodontal Tissue Cells
Objectives: Periodontal disease is an inflammatory condition induced by pathogens that destroy the supporting tissues of the teeth. Allantoin is an anti-inflammatory and wound-healing agent that is widely used in cosmetic and pharmaceutical applications. However, the effects of allantoin on periodontal tissues remain unknown. This study investigates the effects of allantoin on the proliferation and gene expression profile of human gingival fibroblasts (HGFs), and on cytokine production by human THP-1 macrophages.
Methods: HGFs were treated with allantoin concentrations from 0% to 0.05%. After 24 h, mRNA was extracted and purified from the cells and gene expression profiles were evaluated by DNA microarray. Cell proliferation was also assessed using the WST-1 assay. Human THP-1 macrophages were induced to differentiate by phorbol 12-myristate 13-acetate (30 ng/mL, 48 h). These cells were treated with a combination of Porphyromonas gingivalis-derived lipopolysaccharide (LPS; 1 μg/mL) and allantoin (0%–0.05%) for 6 h, followed by measurement of TNF-α production by ELISA.
Results: Allantoin significantly increased cell growth of HGFs in a dose-dependent manner (p<0.01). DNA microarray data revealed that genes associated with cell proliferation were upregulated by allantoin. Furthermore, allantoin significantly decreased LPS-induced TNF-α production by THP-1 macrophages (p<0.01).
Conclusions: These results suggest that allantoin could be useful for gingival tissue repair by promoting the proliferation of gingival cells. Furthermore, allantoin may promote anti-inflammatory responses by supressing the production of inflammatory cyokines by immune cells.
Methods: HGFs were treated with allantoin concentrations from 0% to 0.05%. After 24 h, mRNA was extracted and purified from the cells and gene expression profiles were evaluated by DNA microarray. Cell proliferation was also assessed using the WST-1 assay. Human THP-1 macrophages were induced to differentiate by phorbol 12-myristate 13-acetate (30 ng/mL, 48 h). These cells were treated with a combination of Porphyromonas gingivalis-derived lipopolysaccharide (LPS; 1 μg/mL) and allantoin (0%–0.05%) for 6 h, followed by measurement of TNF-α production by ELISA.
Results: Allantoin significantly increased cell growth of HGFs in a dose-dependent manner (p<0.01). DNA microarray data revealed that genes associated with cell proliferation were upregulated by allantoin. Furthermore, allantoin significantly decreased LPS-induced TNF-α production by THP-1 macrophages (p<0.01).
Conclusions: These results suggest that allantoin could be useful for gingival tissue repair by promoting the proliferation of gingival cells. Furthermore, allantoin may promote anti-inflammatory responses by supressing the production of inflammatory cyokines by immune cells.