The Effect of Fibrin-binding Oligopeptide Derived From Fibronectin on Migration of Periodontal Ligament Cells in an in Vitro Wound Healing Model

Objectives: The aim of this study was to evaluate the effect of synthetic FN fragments containing an N-terminal fibrin-binding domain on migration of human PDL fibroblast cells in an in vitro wound healing model.
Methods: Three types of synthetic oligopeptides, which included fibrin-binding domain of FN (FF1, 3 and 5), were allocated to the experimental groups. Recombinant oligopeptide (F20) including Type III 9-10 domains of FN was used as a positive control and a group treated with nothing was the negative control. Cell migration capacity was evaluated using anCell Migration Assembly kit.
Results: The migration rates and number of migrated cells increased in the test group and both control groups at each point in time. F20, which was used as a positive control in this study, showed a significantly increased cell migration rate as compared with the control group at 6 and 12 h, but not at 18 or 24 h. There were no significant differences among test groups or between the test and positive control (F20) (P<0.05). In examination of the number of migrated cells, there was no observable significance between the test and either control aside from the FF1100μM, and FF5 100μM groups at 6 h and the FF1 50μM, and FF3 100μM groups at 12 h. There was no significant difference among the test groups (P<0.05).
Conclusions: Within the limits of this study, N-terminal fibrin-binding domain of FN promoted cell migration of PDL fibroblast cells.