IADR Abstract Archives
Impact of Plasma Functionalized Graphene on Adhesion and Proliferation of Mesenchymal Stem Cells
Objectives: The aim of the study was to evaluate the impact of oxygen-plasma treated graphene on dental pulp stem cells (DPSC) viability and adhesion. The impact of the surface treatment on the graphene structure was also investigated.
Methods: Graphene grown by chemical vapor deposition was transferred onto glass. Sets were subjected to oxygen plasma for different durations (0s, 5s, 10s, 40s, 60s and 120s). The structure and composition of the modified samples surface were characterised using raman spectroscopy, X-ray photoelectron spectroscopy (XPS), contact angle and atomic force microscopy. DPSC were seeded on the 6 groups of samples as well as non-coated glass and were cultured in common medium. MTS assay was used to evaluate cell viability for 1, 3 and 5 days (n=3). Cell adhesion was investigated through gene expressions for vinculin, talin and beta-integrin for 1 day by RT-CPR (n=3). The statistical analyses were performed with ANOVA.
Results: Covalent bonding of oxygen to carbon atoms induces a conversion from sp2 to sp3 hybridization in the graphene structure. This change was observed by raman spectroscopy (increase of the peaks ratio I(D)/I(G) along with the exposure time) and XPS which confirmed the extent of the functionalization. The contact angle and AFM studies showed respectively an increase in hydrophilicity and surface roughness when the exposure time increases.
All the groups allowed cell survival and proliferation. However, MTS assay and RT-CPR results reflected no significant difference between the 7 groups for each time point.
Conclusions: Oxygen plasma modification of graphene allowed cell survival, proliferation and adhesion to a similar degree than observed on glass and independently of the extent of the functionalization of the graphene.
Methods: Graphene grown by chemical vapor deposition was transferred onto glass. Sets were subjected to oxygen plasma for different durations (0s, 5s, 10s, 40s, 60s and 120s). The structure and composition of the modified samples surface were characterised using raman spectroscopy, X-ray photoelectron spectroscopy (XPS), contact angle and atomic force microscopy. DPSC were seeded on the 6 groups of samples as well as non-coated glass and were cultured in common medium. MTS assay was used to evaluate cell viability for 1, 3 and 5 days (n=3). Cell adhesion was investigated through gene expressions for vinculin, talin and beta-integrin for 1 day by RT-CPR (n=3). The statistical analyses were performed with ANOVA.
Results: Covalent bonding of oxygen to carbon atoms induces a conversion from sp2 to sp3 hybridization in the graphene structure. This change was observed by raman spectroscopy (increase of the peaks ratio I(D)/I(G) along with the exposure time) and XPS which confirmed the extent of the functionalization. The contact angle and AFM studies showed respectively an increase in hydrophilicity and surface roughness when the exposure time increases.
All the groups allowed cell survival and proliferation. However, MTS assay and RT-CPR results reflected no significant difference between the 7 groups for each time point.
Conclusions: Oxygen plasma modification of graphene allowed cell survival, proliferation and adhesion to a similar degree than observed on glass and independently of the extent of the functionalization of the graphene.