IADR Abstract Archives
Regeneration in Post-extraction Sockets Using Autologous Dental Pulp Stem Cells
Objectives: To evaluate the efficacy of autologous dental pulp stem cells carried in collagen sponge, on bone regeneration in post-extraction impacted lower third molar (TMI) sockets.
Methods: A split-mouth, randomized double-blind controlled clinical trial approved by the Local Ethics Committee of Cruces Hospital. Written informed consent was obtained. EUDRACT: 2014-001913-18. The sample size was 30 healthy subjects of both sexes, 18-30 years old, requiring bilateral TMI extractions. One side was randomly chosen as experimental and the other as control. Extractions were in a single session under regional anesthesia, performing full thickness flap, osteotomy, extraction and suture. Once extracted, crowns from roots were separated and the pulp chamber exposed to collect the pulp of both TMI, which was then inserted into a capsule with 1 ml of saline solution. The capsule was placed inside the Rigenera Machine and centrifuged. The obtained cell suspension was incorporated into a collagen membrane (Hemo-klee®) and implanted in the experimental socket. After surgery, a medical grade multislice computer tomography (CT) was performed (Phillis Brilliance 16). The patients were called for a new CT at six months post-surgery. The outcome variable was the volume (mm3) and density (Hounsfield units) of bone regenerated at 6 months post-surgery. Student t-test was used for statistical analysis (level of significance p<0.05).
Results: At the time of writing this abstract, the results of the first two cases have been reported, and no adverse effects were observed.
Control: Volume regenerated [mean (SD)] = -283.2 (315.5) and Density regenerated = 529.2 (555.5)
Experimental: Volume regenerated [mean (SD)] = -294.8 (126.2) and Density regenerated = 666.5 (546.6)
T-test volume and density regenerated: p= 0.97 and p=0.83, respectively.
Conclusions: A tendency towards increased bone density at 6 months in the experimental group has been observed. In order to draw definitive conclusions, the trial needs to continue.
Methods: A split-mouth, randomized double-blind controlled clinical trial approved by the Local Ethics Committee of Cruces Hospital. Written informed consent was obtained. EUDRACT: 2014-001913-18. The sample size was 30 healthy subjects of both sexes, 18-30 years old, requiring bilateral TMI extractions. One side was randomly chosen as experimental and the other as control. Extractions were in a single session under regional anesthesia, performing full thickness flap, osteotomy, extraction and suture. Once extracted, crowns from roots were separated and the pulp chamber exposed to collect the pulp of both TMI, which was then inserted into a capsule with 1 ml of saline solution. The capsule was placed inside the Rigenera Machine and centrifuged. The obtained cell suspension was incorporated into a collagen membrane (Hemo-klee®) and implanted in the experimental socket. After surgery, a medical grade multislice computer tomography (CT) was performed (Phillis Brilliance 16). The patients were called for a new CT at six months post-surgery. The outcome variable was the volume (mm3) and density (Hounsfield units) of bone regenerated at 6 months post-surgery. Student t-test was used for statistical analysis (level of significance p<0.05).
Results: At the time of writing this abstract, the results of the first two cases have been reported, and no adverse effects were observed.
Control: Volume regenerated [mean (SD)] = -283.2 (315.5) and Density regenerated = 529.2 (555.5)
Experimental: Volume regenerated [mean (SD)] = -294.8 (126.2) and Density regenerated = 666.5 (546.6)
T-test volume and density regenerated: p= 0.97 and p=0.83, respectively.
Conclusions: A tendency towards increased bone density at 6 months in the experimental group has been observed. In order to draw definitive conclusions, the trial needs to continue.