IADR Abstract Archives
Recombinant Human Cementum Protein 1 Controlled Releasing Nanoparticles in Electrospun Multiphasic Scaffold for Cementum Regeneration
Objectives: Periodontitis is a major cause for tooth loss which affects around 15% of the adult population. Cementum regeneration has been the crux of constructing the periodontal complex. Cementum protein 1 (CEMP1) is a cementum specific protein which can induce cementogenic differentiation. This study aimed to develop a specific cementum inductive scaffold for clinical application.
Methods: In the present study, poly (ethylene glycol) stabilized amorphous calcium phosphate (ACP) nanoparticles were prepared by wet-chemical method and then loaded with recombinant human CEMP1 (rhCEMP1) for controlled release. An electrospun multiphasic scaffold constituted of poly (ε-caprolactone) (PCL), type-I collagen (COL) and rhCEMP1/ACP were fabricated. The effects of rhCEMP1/ACP/PCL/COL scaffold on the attachment proliferation, osteogenic and cementogenic differentiations of human periodontal ligament cell (PDLC) were systematically investigated. A critical size defect rat model was introduced to evaluate the effect of tissue regeneration of the scaffolds in vivo.
Results: The results showed that poly (ethylene glycol) stabilized ACP nanoparticles formed a core-shell structure with sustained release of rhCEMP1 for up to 4 weeks. rhCEMP1/ACP/PCL/COL scaffold could suppress PDLC proliferation behavior and up-regulate the expression of cementoblastic makers including CEMP1 and cementum attachment protein while down-regulate osteoblastic makers including osteocalcin and osteopontin when it was co-cultured with PDLC in vitro for 7 days. Histology analysis of cementum after being implanted with the scaffold in rats for 8 weeks showed that there was cementum-like tissue formation but few bone formation.
Conclusions: These results indicated the potential of using electrospun multiphasic scaffolds for controlled release of rhCEMP1 for promoting cementum regeneration in reconstruction of the periodontal complex.
Methods: In the present study, poly (ethylene glycol) stabilized amorphous calcium phosphate (ACP) nanoparticles were prepared by wet-chemical method and then loaded with recombinant human CEMP1 (rhCEMP1) for controlled release. An electrospun multiphasic scaffold constituted of poly (ε-caprolactone) (PCL), type-I collagen (COL) and rhCEMP1/ACP were fabricated. The effects of rhCEMP1/ACP/PCL/COL scaffold on the attachment proliferation, osteogenic and cementogenic differentiations of human periodontal ligament cell (PDLC) were systematically investigated. A critical size defect rat model was introduced to evaluate the effect of tissue regeneration of the scaffolds in vivo.
Results: The results showed that poly (ethylene glycol) stabilized ACP nanoparticles formed a core-shell structure with sustained release of rhCEMP1 for up to 4 weeks. rhCEMP1/ACP/PCL/COL scaffold could suppress PDLC proliferation behavior and up-regulate the expression of cementoblastic makers including CEMP1 and cementum attachment protein while down-regulate osteoblastic makers including osteocalcin and osteopontin when it was co-cultured with PDLC in vitro for 7 days. Histology analysis of cementum after being implanted with the scaffold in rats for 8 weeks showed that there was cementum-like tissue formation but few bone formation.
Conclusions: These results indicated the potential of using electrospun multiphasic scaffolds for controlled release of rhCEMP1 for promoting cementum regeneration in reconstruction of the periodontal complex.