IADR Abstract Archives
Anti-cariogenic Properties of a Novel Bioactive Composite
Objectives: To assess the anti-bacterial and anti-cariogenic properties of a novel modified flowable composite enriched with 10%wt. silver-bioglass (Ag-BGCOMP) using an in vitro caries biofilm model.
Methods: Bovine enamel specimens (4X4mm) were used to evaluate Ag-BGCOMP compared to unmodified flowable composite (COMP) in regards to biofilm formation, acidogenicity of the microenvironment, and enamel resistance to demineralization. After restoration with either AG-BGCOMP or COMP, specimens were gas-sterilized and inoculated with Streptococcus mutans and incubated in enriched growth medium for seven days. Growth medium pH was measured daily to determine acid production by the biofilm. At the end of the incubation period, biofilm was stained with disclosing solution and photographed under a stereomicroscope. Then, biofilm was stained with a live-dead staining kit, imaged with a Confocal Microscope (CLSM) and the percentage of live bacteria was assessed using ImageJ software. Enamel demineralization was assessed by surface microhardness (Knoop) at 50, 100, 150, and 250μm from the edge of the restoration. Results were analyzed using one-way ANOVA, Student’s T-test (p<0.05).
Results: The pH culture medium measurements show no significant difference in acidogenicity of S. mutans biofilm between the control (4.76) and Ag-BGCOMP (4.77) groups. Biofilm analyses showed significantly less S. mutans live bacteria on the Ag-BGCOMP specimen (37.3% vs 63.4%). Finally, microhardness results showed less demineralization in the enamel surrounding the restorations with Ag-BGCOMP at all distances, with constant values at each distance. The Average hardness at 250μm was 132.08 for Ag-BGCOMP vs 86.10 for COMP (p<0.0003).
Conclusions: The Ag-BGCOMP exhibited significant antibacterial and anticariogenic properties as demonstrated by Ag-BGCOMP’s ability to decrease S. mutans live bacteria and by its ability to protect the enamel adjacent to the material from the demineralization process. Ag-BGCOMP may provide a powerful new option for high caries risk patients.
Methods: Bovine enamel specimens (4X4mm) were used to evaluate Ag-BGCOMP compared to unmodified flowable composite (COMP) in regards to biofilm formation, acidogenicity of the microenvironment, and enamel resistance to demineralization. After restoration with either AG-BGCOMP or COMP, specimens were gas-sterilized and inoculated with Streptococcus mutans and incubated in enriched growth medium for seven days. Growth medium pH was measured daily to determine acid production by the biofilm. At the end of the incubation period, biofilm was stained with disclosing solution and photographed under a stereomicroscope. Then, biofilm was stained with a live-dead staining kit, imaged with a Confocal Microscope (CLSM) and the percentage of live bacteria was assessed using ImageJ software. Enamel demineralization was assessed by surface microhardness (Knoop) at 50, 100, 150, and 250μm from the edge of the restoration. Results were analyzed using one-way ANOVA, Student’s T-test (p<0.05).
Results: The pH culture medium measurements show no significant difference in acidogenicity of S. mutans biofilm between the control (4.76) and Ag-BGCOMP (4.77) groups. Biofilm analyses showed significantly less S. mutans live bacteria on the Ag-BGCOMP specimen (37.3% vs 63.4%). Finally, microhardness results showed less demineralization in the enamel surrounding the restorations with Ag-BGCOMP at all distances, with constant values at each distance. The Average hardness at 250μm was 132.08 for Ag-BGCOMP vs 86.10 for COMP (p<0.0003).
Conclusions: The Ag-BGCOMP exhibited significant antibacterial and anticariogenic properties as demonstrated by Ag-BGCOMP’s ability to decrease S. mutans live bacteria and by its ability to protect the enamel adjacent to the material from the demineralization process. Ag-BGCOMP may provide a powerful new option for high caries risk patients.