IADR Abstract Archives
Therapeutic Implications of Propolis and Curry Leaf Against Periodontitis
Objectives: In in vitro screening of a wide range of natural products, propolis, a honeybee product, and curry leaf, an herbal product, were found to be potential antimicrobials against periodontitis. In the present study, we examined whether the topical administration reduces the amounts of periodontopathic bacteria and further improves periodontal parameters.
Methods: Twenty four subjects with moderate or severe chronic periodontitis were randomly allocated to four groups with different paste treatments as follows: propolis, curry leaf, tetracycline, and placebo. After completion of basic periodontal therapy, each paste was administrated into a periodontal pocket defined as target for 3 times at 1 month intervals. Saliva and gingival crevicular fluid (GCF) samples were collected just prior to first intervention (baseline) and 1 month after final intervention. All subjects except 1 dropped out (in curry leaf group) were evaluated for a range of analyses. Numbers of a series of periodontopathic bacteria were quantified by TaqMan real-time PCR. Changes in several clinical parameters from baseline to after intervention were also analyzed.
Results: Five of 6 subjects who were allocated in propolis group were P. gingivalis-positive in GCF, however, 3 of the 5 P. gingivalis-positive patients decreased the numbers of P. gingivalis to below the detection limit of real-time PCR. In saliva, all 5 subjects of curry leaf group decreased the numbers of P. gingivalis after the interventions, while all 6 subjects of tetracycline group increased the numbers of total oral bacteria after the interventions. There was a tendency toward improvement of bleeding on probing in propolis and tetracycline groups compared to curry leaf and placebo groups. The probing pocket depth of propolis group significantly decreased after intervention compared to the baseline, but not after the other interventions (Fig. 1).
Conclusions: We suggest that propolis and curry leaf are potential candidates for complementary and alternative medications against periodontitis.
Methods: Twenty four subjects with moderate or severe chronic periodontitis were randomly allocated to four groups with different paste treatments as follows: propolis, curry leaf, tetracycline, and placebo. After completion of basic periodontal therapy, each paste was administrated into a periodontal pocket defined as target for 3 times at 1 month intervals. Saliva and gingival crevicular fluid (GCF) samples were collected just prior to first intervention (baseline) and 1 month after final intervention. All subjects except 1 dropped out (in curry leaf group) were evaluated for a range of analyses. Numbers of a series of periodontopathic bacteria were quantified by TaqMan real-time PCR. Changes in several clinical parameters from baseline to after intervention were also analyzed.
Results: Five of 6 subjects who were allocated in propolis group were P. gingivalis-positive in GCF, however, 3 of the 5 P. gingivalis-positive patients decreased the numbers of P. gingivalis to below the detection limit of real-time PCR. In saliva, all 5 subjects of curry leaf group decreased the numbers of P. gingivalis after the interventions, while all 6 subjects of tetracycline group increased the numbers of total oral bacteria after the interventions. There was a tendency toward improvement of bleeding on probing in propolis and tetracycline groups compared to curry leaf and placebo groups. The probing pocket depth of propolis group significantly decreased after intervention compared to the baseline, but not after the other interventions (Fig. 1).
Conclusions: We suggest that propolis and curry leaf are potential candidates for complementary and alternative medications against periodontitis.
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