The Effect of Inflammation on Proliferation and Osteogenic Differentiation of Human PDLCs
Objectives: This study was to investigate the effects of inflammatory microenvironment on proliferation and the osteogenic differentiation of human periodontal ligament cells (PDLCs) in vitro. Methods: Human PDLCs were isolated and characterized. The proliferation rate of PDLCs under different concentration of lipopolysaccharide（LPS） was tested by MTT assay. The PDLCs’ osteogenic differentiation was investigated using real-time PCR and western-blot. The date was statistically analyzed with SPSS13.0 software package. Results: Immunocytochemical staining verified that the isolated cells were PDLCs. The proliferation of PDLCs and the expression of alkaline phosphatase (ALP), RUNX2, Collagen I, BMP2 were significantly decreased by10ug/ml LPS. Conclusions: The inflammatory microenvironment inhibits the proliferation and osteogenic differentiation of human PDLCs.
Division: IADR/APR General Session
Meeting:2016 IADR/APR General Session (Seoul, Korea) Location: Seoul, Korea
Year: 2016 Final Presentation ID:0766 Abstract Category|Abstract Category(s):Periodontal Research-Diagnosis/Epidemiology
Authors
Song, Zhongchen
( Shanghai Jiao Tong University
, Shanghai
, China
)
Shu, Rong
( Ninth People's Hospital, SJTU
, Shanghai
, China
)
Financial Interest Disclosure: The authors reported no conflicts of interest related to this study.