Zinc Finger and BTB Domain-containing 20 (ZBTB20) Regulates Osteo/odontogenic Differentiation of HDPSCs via the NF-κB Pathway

Objectives: Multiple factors and signaling molecules regulate odontogenic differentiation of human dental pulp stem cells (HDPSCs). Their regulatory mechanisms are not completely understood. In our study, the role of Zinc finger and BTB domain-containing 20 (ZBTB20) in odontoblastic differentiation of HDPSCs was investgated.
Methods: HDPSCs were obtained from human third molars. Then ZBTB20 expression was examined by qRT-PCR and western blot. Their osteo/odontogenic differentiation and the involvement of NF-κB pathway were subsequently investigated.
Results: The expression of ZBTB20 is upregulated in a time-dependent manner during odontogenic differentiation of hDPSCs. Inhibition of ZBTB20 reduced osteogenic medium (OM)-induced odontogenic differentiation, reflected in decreased alkaline phosphatase (ALP) activity, mineralized nodule formation and mRNA expression of odonto/osteogenic marker genes. In contrast, overexpression of ZBTB20 enhanced ALP activity, mineralization and the expression of differentiation marker genes. Furthermore, the expression of IκBα was increased by ZBTB20 silencing in HDPSCs, whereas ZBTB20 overexpression decreased IκBα and enhanced nuclear NF-κB p65. Inhibition of the NF-κB pathway significantly suppressed the odontogenic differentiation of HDPSCs induced by ZBTB20.
Conclusions: This study shows that ZBTB20 plays an important role during odontoblastic differentiation of HDPSCs and may have clinical implications for regenerative endodontics for the first time.