Effect of Plaque Removal Mechanism and Duration on Oral Biofilm
Objectives: Dental plaque contributes to oral and systemic health concerns, providing impetus for more effective approaches to oral hygiene. Aim of this study was to compare plaque removal using 2 brushing durations and 2 toothpaste formulations with different mechanistic concepts. The test gel (Livionex Dental GelR Los Gatos, CA) disrupts biofilm through metal chelation, eschewing traditional abrasives, detergents, and anti-microbials. Aquafresh Deep ActionR (GSK, Brentford, UK) served as control. Methods: Ten subjects participated in this double-blinded, randomized, cross-over study. Subjects were photographed and plaque levels documented (Quigley-Hein Plaque Index (PI)). Volunteers brushed with washout toothpaste (Tom’s, Kennebunk, ME) twice daily for 7 days. On the evening of Day 7 subjects used the assigned study dentifrice, then abstained from oral hygiene and food until their appointment the next morning. After PI measurement, subjects rinsed with 2-Tone (Young Dental, Aurora, IL) disclosing solution, and photographs were recorded. Subjects brushed with the same toothpaste as the previous night for 1 or 2 minutes and measurements were repeated. All subjects used both toothpastes and both cleaning durations with a 1-week washout between each of the 4 legs of the study. Photographs were analyzed using Image-J software to determine plaque stain area. Results: After one-time control toothpaste use in the evening, PI the next morning averaged 1.2 (SD 0.18) with 36.1% of tooth surfaces covered by new plaque. Using the test compound, morning PI averaged 0.4 (SD 0.06) with 26.5% of tooth surfaces covered with new plaque (Sig p<0.05). Subsequent toothbrushing with the test toothpaste reduced plaque by 87% (S.D. 11) for the 1 and 2 minute durations (NSig p>0.05). Using the control formulation, plaque was reduced by 83% (S.D. 12) after 2 minutes and by 77% (S.D. 11) after 1 minute (Sig p<0.05). Conclusions: Metal chelation may discourage plaque accumulation and support biofilm removal.
Division: IADR/AADR/CADR General Session
Meeting:2015 IADR/AADR/CADR General Session (Boston, Massachusetts) Location: Boston, Massachusetts
Year: 2015 Final Presentation ID:3079 Abstract Category|Abstract Category(s):Oral Health Research
Authors
Sahni, Karan
( University of California, Irvine
, Irvine
, California
, United States
)
Sarraf, Amin
( University of California, Irvine
, Irvine
, California
, United States
)
Chow, Brian
( University of California, Irvine
, Irvine
, California
, United States
)
Nazeri, Erfan
( University of California, Irvine
, Irvine
, California
, United States
)
Caro, Christoffer
( University of California, Irvine
, Irvine
, California
, United States
)
Chan, Gregory
( University of California, Irvine
, Irvine
, California
, United States
)
Wilder-smith, Petra
( University of California, Irvine
, Irvine
, California
, United States
)
Support Funding Agency/Grant Number: Livionex Dental Gel, Los Gatos, CA. The project described was supported by award number P41EB015890 from the National Institute of Biomedical Imaging and Bioengineering. The content is solely the responsibility of the authors and does not necessarily repr
Financial Interest Disclosure: NONE