IADR Abstract Archives
Effects of Dynamatrix® on Odontogenic Differentiation of Human Pulp Cells
Objectives: Objective: Dynamatrix®, a collagen based scaffold manufactured from pig small intestine basal membrane after decellularization process, contains collagen (Types I, III, VI), glycosaminoglycans (hyaluronic acid, chondroitin sulfate A and B, heparin, and heparan sulfate), proteoglycans, growth factors (FGF-2, TGF-β), and fibronectin. It has often been used in periodontal defect treatment. Based on its biological nature, we believe that Dynamatrix® has the great potential to be applied in the regenerative endodontics as a support for cell attachment and growth, and as a reservoir to provide growth factors to the local stem cells for their proliferation and differentiation. Our current study is to evaluate the induction ability of Dynamatrix® on odontogenic differentiation of four different human dental pulp cells.
Methods: Methodology: Acquired from natal teeth, children deciduous teeth, and orthodontic extracted permanent teeth with institutional IRB approvals, human natal pulp cells (passage 3-9), pulp cells from human exfoliated deciduous teeth (passage 5-8), and human adult dental pulp cells (passage 3-8), as well as a commercial human dental pulp stem cell line (DPSCs, passage 3-8) purchased from Cook Biotech (West Lafayette, IN) were used in this study. Each cell line was cultured with or without Dynamatrix® membrane in 6-well plates for 24 hours with serum- media before the total RNA was collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of odontoblast proteins, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Human type I collagen, the primary extracellular matrix protein in dentin, was also detected in these cell lines. Cyclophilin was used as the internal control for a semi-quantitative analysis on the density of the mRNA expression in each cell line. Each experiment was repeated at least 3 times. A one-way ANOVA test will be performed.
Results: Results and Conclusion: Dynamatrix® can alter the mRNA expression of odontoblast proteins DSP and DPP
Conclusions: Results and Conclusion: Dynamatrix® can alter the mRNA expression of odontoblast proteins DSP and DPP
Methods: Methodology: Acquired from natal teeth, children deciduous teeth, and orthodontic extracted permanent teeth with institutional IRB approvals, human natal pulp cells (passage 3-9), pulp cells from human exfoliated deciduous teeth (passage 5-8), and human adult dental pulp cells (passage 3-8), as well as a commercial human dental pulp stem cell line (DPSCs, passage 3-8) purchased from Cook Biotech (West Lafayette, IN) were used in this study. Each cell line was cultured with or without Dynamatrix® membrane in 6-well plates for 24 hours with serum- media before the total RNA was collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of odontoblast proteins, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Human type I collagen, the primary extracellular matrix protein in dentin, was also detected in these cell lines. Cyclophilin was used as the internal control for a semi-quantitative analysis on the density of the mRNA expression in each cell line. Each experiment was repeated at least 3 times. A one-way ANOVA test will be performed.
Results: Results and Conclusion: Dynamatrix® can alter the mRNA expression of odontoblast proteins DSP and DPP
Conclusions: Results and Conclusion: Dynamatrix® can alter the mRNA expression of odontoblast proteins DSP and DPP