IADR Abstract Archives
Antiplaque Effect and Substantivity of Hypochlorous Acid Rinses for Use in the Oral Cavity. A Pilot Study
Objectives: The hypochlorous acid (HOCl) in a non-antibiotic antimicrobial agent used in clinical medicine but its antiplaque effect has not been assessed. The aim of this study was to compare the effect of a rinse of HOCl in plaque control compared with placebo and CHX, assess the substantivity and adverse effects.
Methods: A double-blind randomized clinical trial in 60 participants with good oral status was performed. They were randomized into five groups: HOCl 0.025% and 0.05%, CHX 0.12% and 0.2% and placebo. Participants were instructed to rinse with 10 ml of each solution for 30sec after a dental prophylaxis. Saliva samples were taken at baseline and after 30sec, 1, 3, 5 and 7hrs to evaluate the substantivity. To establish the bacterial viability a fluorescence kit(Live/DEAD® BacLight) was used, and microscopy captures were digitalized by AxioVision LE 4.8 software. All participants were assessed with Turesky visible plaque index at follow-up. The adverse events were evaluated. Analyzes were performed by ANOVA, paired T test and Chi2/Fisher exact test (p<0.05).
Results: Placebo showed the highest average plaque after 7hrs 1.37±0.5. HOCl groups 0.025% and 0.05% showed lower plaque counts 1.19±0.42 and 1.12±0.25 respectively. CHX rinses showed similar results to those of HOCl; at 0.12%, 1.27±0.25 and 0.2% 1.11±0.45. HOCl showed a reduction in bacterial viability after 30sec (p<0.05), but not substantivity over time. Rinsing HOCl 0.05% showed a statistically significant relative to the sense of taste. CHX showed higher burning sensation of mucous after rinsing.
Conclusions: A good performance of HOCl 0.025% and 0.05% was observed as initial plaque formation despite not show substantivity. With the findings of this study is possible to continue clinical studies to longer term to assess its bioequivalence with CHX as antiplaque agent.
Methods: A double-blind randomized clinical trial in 60 participants with good oral status was performed. They were randomized into five groups: HOCl 0.025% and 0.05%, CHX 0.12% and 0.2% and placebo. Participants were instructed to rinse with 10 ml of each solution for 30sec after a dental prophylaxis. Saliva samples were taken at baseline and after 30sec, 1, 3, 5 and 7hrs to evaluate the substantivity. To establish the bacterial viability a fluorescence kit(Live/DEAD® BacLight) was used, and microscopy captures were digitalized by AxioVision LE 4.8 software. All participants were assessed with Turesky visible plaque index at follow-up. The adverse events were evaluated. Analyzes were performed by ANOVA, paired T test and Chi2/Fisher exact test (p<0.05).
Results: Placebo showed the highest average plaque after 7hrs 1.37±0.5. HOCl groups 0.025% and 0.05% showed lower plaque counts 1.19±0.42 and 1.12±0.25 respectively. CHX rinses showed similar results to those of HOCl; at 0.12%, 1.27±0.25 and 0.2% 1.11±0.45. HOCl showed a reduction in bacterial viability after 30sec (p<0.05), but not substantivity over time. Rinsing HOCl 0.05% showed a statistically significant relative to the sense of taste. CHX showed higher burning sensation of mucous after rinsing.
Conclusions: A good performance of HOCl 0.025% and 0.05% was observed as initial plaque formation despite not show substantivity. With the findings of this study is possible to continue clinical studies to longer term to assess its bioequivalence with CHX as antiplaque agent.