IADR Abstract Archives
Analysis of a Complex Oral Biofilm on Abutments Implants Surfaces
Objectives: Dental implant rehabilitation has become a routine procedure to replace missing teeth. Its success depends on proper osseointegration, as well as the lack of inflammation. An important factor contributing to the development of inflammation is the attachment of microorganisms and subsequent development of biofilms on the prosthetic components. The aim of this investigation was to compare the in vitro development of a complex oral biofilm on two different abutments implants surfaces, titanium and zirconia.
Methods: Pure titanium (T) and zirconia stabilized with yttrium (ZrO2) disks were used as experimental groups, and bovine enamel disks as a positive control. Roughness and surface energy of all surfaces was analyzed. Biofilm development was performed by incubating the disks in rich medium containing a complex representative saliva-derived oral microbial community for 16 and 48 hours. The bacterial profile was evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) and the dominant bands were excised and sequenced for genus identification.
Results: We found similarity on bacteria profile after 16 hours, but the time affected the dominance of some bacteria species after 48 hours incubation. We identify Porphyromonas species strongly adhered on T disks.
Conclusions: These new findings suggest that the material composition has an important impact on the type of bacteria that adhered onto abutment surfaces. This information can lead to important development in strategies to create anti-microbial titanium surfaces, leading to significant improvement in implant longevity.
Methods: Pure titanium (T) and zirconia stabilized with yttrium (ZrO2) disks were used as experimental groups, and bovine enamel disks as a positive control. Roughness and surface energy of all surfaces was analyzed. Biofilm development was performed by incubating the disks in rich medium containing a complex representative saliva-derived oral microbial community for 16 and 48 hours. The bacterial profile was evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) and the dominant bands were excised and sequenced for genus identification.
Results: We found similarity on bacteria profile after 16 hours, but the time affected the dominance of some bacteria species after 48 hours incubation. We identify Porphyromonas species strongly adhered on T disks.
Conclusions: These new findings suggest that the material composition has an important impact on the type of bacteria that adhered onto abutment surfaces. This information can lead to important development in strategies to create anti-microbial titanium surfaces, leading to significant improvement in implant longevity.