IADR Abstract Archives
Effect of Fluoride-varnish And CPP-ACPF-containing-varnish on Microhardness of Demineralized Enamel
Objectives: Dental caries which is caused by tooth structure demineralization remains a significant public health problem especially in orthodontic patients whose oral hygiene is impaired. The demineralization process can be arrested or reversed by mineralizing materials. The aim of this in vitro study was to determine the effect of fluoride and CPP-ACPF-containing varnishes on microhardness of demineralized enamel.
Methods: Thirty sectioned pieces (3×3mm) were prepared from sound extracted human premolars and evaluated for microhardness values with Vicker’s microhardness tester (50gr, 15s). The base line microhardness test was done and then the samples were immersed in demineralizing gel for 96 hours and evaluated for microhardness test again. Thereafter the samples were randomly assigned into three groups of 10 as follow: Group1: (control) no varnish applied and samples remained in artificial saliva for four weeks. Group 2: Fluoride varnish (DuraShield, Sultan Health care inc., USA) was applied three times a week for four weeks. Group 3: CPP-ACPF-containing varnish (MI Varnish, GC, USA) was used with the same protocol as G2. The final microhardness test was done after four weeks. To evaluate the possible relation between the Diagnodent measurments and microhardness values, all samples were evaluated by Diagnodent (Kavo, USA) at the same time of microhardness test. Data were analyzed with repeated measure ANOVA and Post Hock Tukey HSD test with 95% confidence level.
Results: Both Fluoride varnish and CPP-ACPF-containing varnish significantly increased the Vicker's hardness number of demineralized enamel (p<0.05). However, according to the Tukey HSD test, the ability of CPP-ACPF-containing varnish to remineralize the area was significantly better (p<0.05). There was no relation between Diagnodent numbers and microhardness values.
Conclusions: Fluoride and CPP-ACPF varnishes could increase the hardness of demineralized enamel. However, CPP-ACPF containing varnish showed significantly superior results.
Methods: Thirty sectioned pieces (3×3mm) were prepared from sound extracted human premolars and evaluated for microhardness values with Vicker’s microhardness tester (50gr, 15s). The base line microhardness test was done and then the samples were immersed in demineralizing gel for 96 hours and evaluated for microhardness test again. Thereafter the samples were randomly assigned into three groups of 10 as follow: Group1: (control) no varnish applied and samples remained in artificial saliva for four weeks. Group 2: Fluoride varnish (DuraShield, Sultan Health care inc., USA) was applied three times a week for four weeks. Group 3: CPP-ACPF-containing varnish (MI Varnish, GC, USA) was used with the same protocol as G2. The final microhardness test was done after four weeks. To evaluate the possible relation between the Diagnodent measurments and microhardness values, all samples were evaluated by Diagnodent (Kavo, USA) at the same time of microhardness test. Data were analyzed with repeated measure ANOVA and Post Hock Tukey HSD test with 95% confidence level.
Results: Both Fluoride varnish and CPP-ACPF-containing varnish significantly increased the Vicker's hardness number of demineralized enamel (p<0.05). However, according to the Tukey HSD test, the ability of CPP-ACPF-containing varnish to remineralize the area was significantly better (p<0.05). There was no relation between Diagnodent numbers and microhardness values.
Conclusions: Fluoride and CPP-ACPF varnishes could increase the hardness of demineralized enamel. However, CPP-ACPF containing varnish showed significantly superior results.
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