IADR Abstract Archives
Antifungic Activity Monitoring in Patients With Persistent Apical Periodontitis
Objectives: The objective of this study was assess, in vitro, the antifungic activity of the tea-tree oil (TTO) both by contact and from a distance against yeasts obtained from a patient with persistent apical peritonitis (PAP).
Methods: Yeasts collected from a patients with PAP were isolated and identified, the susceptibility profile in relation to the antifungic fluconazole, itraconazole, amphotericin B, and TTO was assessed by the broth micro-dilution test. The antifungic activity of the TTO was also assessed by contact, by the disk diffusion test, and from a distance, by a modified protocol using extracted human teeth, comparing its effects to those of tricresolformaline.
Results: Candida albicans and Candida tropicalis were identified, and their minimum inhibitory concentrations (MIC) for TTO were 190 µg/mL and 390 µg/mL,respectively. Both medicaments produced inhibition halos by contact. TTO presented an increasing antifungic activity from a distance at 24 and 48 h, whereas tricresolformaline inhibited growth completely at 24 h.
Conclusions: The results of this study demonstrated that TTO has antifungic properties that qualify it as a potential intracanal medicament.
Key Words: Tea-Tree oil, periapical periodontitis, volatilization, antifungic activity.
Methods: Yeasts collected from a patients with PAP were isolated and identified, the susceptibility profile in relation to the antifungic fluconazole, itraconazole, amphotericin B, and TTO was assessed by the broth micro-dilution test. The antifungic activity of the TTO was also assessed by contact, by the disk diffusion test, and from a distance, by a modified protocol using extracted human teeth, comparing its effects to those of tricresolformaline.
Results: Candida albicans and Candida tropicalis were identified, and their minimum inhibitory concentrations (MIC) for TTO were 190 µg/mL and 390 µg/mL,respectively. Both medicaments produced inhibition halos by contact. TTO presented an increasing antifungic activity from a distance at 24 and 48 h, whereas tricresolformaline inhibited growth completely at 24 h.
Conclusions: The results of this study demonstrated that TTO has antifungic properties that qualify it as a potential intracanal medicament.
Key Words: Tea-Tree oil, periapical periodontitis, volatilization, antifungic activity.