Effects of Ovariectomy on Periodontal Tissues Following Tooth Replantation

Objectives: The aim of the study was analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation through histomorphometric, immunohistochemistry and μ-CT analysis in rats.
Methods: Eighty Wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: Group ovariectomized (OVX), and Sham group. After two months, the rats were followed by extraction of upper right incisor and immediate replantation. The rats were euthanized 28, 45 and 60 days after tooth reimplantation. Histomorphometric and immunohistochemical analysis was performed by evaluation of PCNA and TRAP proteins. For μ-CT it was considered the early postoperative time 28 days (n = 10) and the final postoperative time 60 days (n = 10). The volumetric data allowed to evaluated and quantify the ​​root resorption extention, the periodontal ligament space, the bone resorption and depth of root resorption. All the data were analyzed by ANOVA and underwent to the nonparametric Mann-Whitney test. The significance level was 5%.
Results: Periodontal ligament was reinserted in the bone and cementum and the immunohistochemical analysis revealed PCNA expression in the periodontal ligament independent of the group. There was no statistically significant difference between the groups for the PCNA and TRAP proteins. The histomorphometric analysis showed statistically significant difference between groups in the histomorphological details of periodontal ligament and root resorption. The μ-CT analysis showed multiple areas of bone resorption in association with OVX 30 and 60 days without statistically significant difference between groups.
Conclusions: In conclusion, the ovariectomy did not have significant influence in immediate tooth replantation repair.