IADR Abstract Archives
Local Drug Release and Biological Evaluation of Antimicrobial Peptide-loaded Coatings using a Layer-by-Layer Process on Titanium
Objectives: The purpose was to establish a coating with sustained antimicrobial activity on titanium surface.
Methods: The antimicrobial peptide Tet213 was grafted to collagen Ⅳ.The multilayer AMPCol coatings were assembled on smooth titanium surfaces using a layer-by-layer technique. The assembly process and the physicochemical properties were characterized using XPS, AFM, contact angle, QCM. Cytotoxicity, erythrocyte hemolysis and antimicrobial activities were observed.
Results: A multilayer coating containing an antimicrobial peptide was fabricated on smooth titanium via the LBL assembly process. The AMPCol-loaded layers form a thin hydrophilic film with a controlled thickness. Attachment of HaCaT cells to the coated titanium plates was observed and noncytotoxicity was found. Low erythrocyte hemolysis was observed on all coated plates, which indicated high hemocompatibility. A sustained release assay and antimicrobial tests show that the titanium plate with AMPCol coating decreased the growth of both a Gram-positive aerobe (Staphylococcus aureus) and a Gram-negative anaerobe (Porphyromonas gingivalis) up to one month. Early S. aureus biofilm formation was inhibited by the coating.
Conclusions: Through LBL technique, a non-cytotoxic multilayered coating, which had the excellent long-term sustained antimicrobial activity, was built on smooth titanium surface. This will be a potential method for preventing peri-implantitis.
Methods: The antimicrobial peptide Tet213 was grafted to collagen Ⅳ.The multilayer AMPCol coatings were assembled on smooth titanium surfaces using a layer-by-layer technique. The assembly process and the physicochemical properties were characterized using XPS, AFM, contact angle, QCM. Cytotoxicity, erythrocyte hemolysis and antimicrobial activities were observed.
Results: A multilayer coating containing an antimicrobial peptide was fabricated on smooth titanium via the LBL assembly process. The AMPCol-loaded layers form a thin hydrophilic film with a controlled thickness. Attachment of HaCaT cells to the coated titanium plates was observed and noncytotoxicity was found. Low erythrocyte hemolysis was observed on all coated plates, which indicated high hemocompatibility. A sustained release assay and antimicrobial tests show that the titanium plate with AMPCol coating decreased the growth of both a Gram-positive aerobe (Staphylococcus aureus) and a Gram-negative anaerobe (Porphyromonas gingivalis) up to one month. Early S. aureus biofilm formation was inhibited by the coating.
Conclusions: Through LBL technique, a non-cytotoxic multilayered coating, which had the excellent long-term sustained antimicrobial activity, was built on smooth titanium surface. This will be a potential method for preventing peri-implantitis.