Triclosan Microparticles for Antifungal Denture Bases

Objectives: Add triclosan-microparticles to three commercial acrylic-resins to evaluate its antifungal effect and cytotoxicity.
Methods: Triclosan-microparticles were analyzed by Scanning Electron Microscopy. Three acrylic-resins were used: OptiCryl^®(conventional-thermopolymerization), Nature-Cryl^® and EZ-Cryl^® (microwave-polymerization). Two groups of each acrylic-resin were performed; the first by following the manufacturer’s-instruction and the second by adding triclosan-microparticles (3%). The antifungal assay was performed by the preparation of disks (n=10) of 10mmx2mm in size of each acrylic-resin. Candida albicans cultures were prepared of 2x10⁵ CFU/ml and placed in 24-well plates. Each sample were placed in the culture (48h, 37ºC); after that, the Candida solution was placed in an authomatic counter to know the difference of Colony-Forming Unit, to assess the antifungal effect of each group. The positive control was Candida culture with fungizone. The cytotoxicity was evaluated by the MTT-assay using mouse fibroblast cell-line at 24h. One-Way ANOVA was applied.
Results: The micrography showed irregular-large particles of Triclosan with 15x50micrometers in size. The results for the antifungal evaluation were: OptiCryl^® and Nature-Cryl^® presented the lower Candida proliferation (1.7x10⁵CFU), showing significant difference (p<0.05) to EZ-Cryl^® with (2.1x10⁵CFU). The groups with triclosan presented the same behavior but with different values: OptiCryl^® and Nature-Cryl^® presented the lower Candida albicans proliferation (1.6 and 1.4x10⁵CFU-respectively), showing significant difference (p<0.05) to EZ-Cryl^® with (2.0x10⁵CFU). The MTT-assay showed no significant difference (p>0.05) for all-groups.
Conclusions: The addition of tricosan-microparticles (3%) decrease the Candida albicans proliferation in OptiCryl^® and Nature-Cryl^® and they were no-cytotoxic to cultures cells, indicating that Triclosan is a well antifungal agent when used in this proportion for denture bases.Acknowledgements: Projects (DGAPA-UNAM): PAPIIT-TA200414, PAPIME-PE202214; CONACyT-Mexico(CB176450), Dra. Genoveva Hernández-Padrón and Dra. Marina Vega.