Effect of Artificial Saliva on Transcriptome of Proinflammatory Cytokines and Chemokynes

Objectives: Artificial saliva (AS) is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. The aim of this study was to determine if components of commercially available artificial saliva are responsible for the proinflammatory response in normal adult human oral fibroblasts (HGF1) as a model in vitro.
Methods: 3% concentration of human saliva was used as negative control and the same concentration was used for AS group for 24 hours of exposition. To determine the effects of AS on gene expression, a qRT-PCR array analysis were performed. Gene expression was determined by Qiagen software.
Results: The expression of C3, CCL19, CXCL1, CXCL2, CXCL3, CXCL5, CXCL6 (Regulation of Inflammatory Response), CCL5 (Chronic Inflammatory Response), NFKB1 (Humoral Immune Response), IL6, IL8 (Proinflammatory Interleukins) and IL6R, IL1RN (Acute-Phase Response) were downregulated by AS treatments.
Conclusions: Our findings demonstrate that the exposure of HGF1 to AS modulate the inflammatory response of these cells when compared to human saliva.