Objectives: It is estimated at least one painful symptom of orofacial pain is related to temporomandibular join (TMJ) region. Alternative therapies, such as low-level laser (LLLT), is used to minimize the medications use, but the specific dose and the events caused still need to be investigated. Methods: Male rats received administration of Freund's Adjuvant (CFA) or saline 0.9%(SAL) into TMJ (50µL), and were treated or not (day 1, 3, 7 and 10) with LLLT (20 or 10J/cm2) (CEUA protocol 2013.1.1111.58.7). In synovial fluid was evaluated the reactive oxygen species by Myeloperoxidase analysis (MPO) and the type of metalloproteinase by the conventional zymography. The histology of TMJ was performed and polymorphonuclear cells were investigated through of H&E. Two-way ANOVA was performed followed by post hoc test Newman-Keuls. Results: To MPO the analysis showed to 10J/cm2 dose there were difference in time (P<0.01) and treatment (P<0.01) and interaction between them (P<0.01). To 20 J/cm2 dose was evidenced difference in time (P<0.01), treatment (P<0.01) and interaction between them (P<0.01) when compared CFA and other groups. The H&E analysis showed to 10J/cm2 dose difference in treatment (P<0.05). Moreover to 20 J/cm2 dose was evidenced difference in treatment (P<0.01) when compared CFA and the other groups. Additionally, MMP-9(95kDa) has significant difference between time (P<0.01), treatment (P<0.01) and interaction time-treatment (P<0.05). The MMP-2(72KDa), showed difference in time (P<0.01) and treatment (P<0.01) and MMP-2(75KDa) has difference between time (P<0.01) and treatment (P<0.01). MMP-2(64KDa) showed a difference among time (P<0.01), treatment (P<0.01) and interaction between them (P<0.01). Relative to 10J/cm2 dose, MMP-9 showed difference in treatment (P<0.01). Moreover, MMP-2 exhibited difference in the treatment for the 64kDa (P<0.05), 72kDa (P<0.01) and 75kDa (P<0.01). The difference verified all analysis occurred between SHAM+CFA and other groups. Conclusions: The most effective dose tested to TMJ inflammation was 20J/cm2.
Division: IADR/AADR/CADR General Session
Meeting:2015 IADR/AADR/CADR General Session (Boston, Massachusetts) Location: Boston, Massachusetts
Year: 2015 Final Presentation ID:2710 Abstract Category|Abstract Category(s):Oral Medicine & Pathology
Authors
Desiderá, Amanda
( Dentistry School of Ribeirao Preto, University of Sao Paulo
, Ribeirao Preto
, Sao Paulo
, Brazil
)
Martins, Amanda
( Dentistry School of Ribeirao Preto, University of Sao Paulo
, Ribeirao Preto
, Sao Paulo
, Brazil
)
Do Nascimento, Glauce
( Faculty of Phylosophy Sciences and Letter of Ribeirao Pretos
, Ribeirao Preto
, Brazil
; Dentistry School of Ribeirao Preto, University of Sao Paulo
, Ribeirao Preto
, Sao Paulo
, Brazil
)
Gerlach, Raquel
( Dentistry School of Ribeirao Preto, University of Sao Paulo
, Ribeirao Preto
, Sao Paulo
, Brazil
)
Leite-panissi, Christie
( Dentistry School of Ribeirao Preto, University of Sao Paulo
, Ribeirao Preto
, Sao Paulo
, Brazil
; Faculty of Phylosophy Sciences and Letter of Ribeirao Pretos
, Ribeirao Preto
, Brazil
)
Support Funding Agency/Grant Number: FAPESP process 2013/15588-3.
Financial Interest Disclosure: AC Desiderá has a Phd’s degree scholarship from FAPESP (# 2013/15588-3). GC Nascimento has a PhD’s degree scholarship from FAPESP (#2011/07718-9). CRA Leite-Panissi received research grants from the CNPq (# 307383/2012-1).