IADR Abstract Archives

Effect of Material Surfaces on Bacterial Adherence

Objectives: This study compared Streptococcus gordonii adherence to saliva-coated surfaces of enamel (E) and dental materials amalgam (A, Tytin), composite (C, Estelite Sigma Quick), lithium disilicate (LD, IPS e.max), and zirconia (Z, Y-TZP) with and without wear of the surfaces.
Methods: Six sample specimens from each material were prepared and underwent 240,000 cycles of wear with 5 kg (49 N) chewing load with zirconia antagonists in a dual-axis chewing simulator. Specimens were sterilized under UV light and then immersed in filtered saliva collected from one donor. After washing with Phosphate-Buffered Saline (PBS), specimens were placed in individual wells containing 1 x 108 cells/ml of S. gordonii in PBS. Immersed specimens were incubated at 30° C for 2h and washed twice again with PBS, left in Karnovsky’s fixative for 24h, dehydrated, coated with gold-palladium, and examined under SEM. Four images (x 5000) of each sample were taken from worn and unworn surfaces and numbers of the bacteria were counted on the images. Mean values were calculated. ANOVA and post hoc Tukey’s test were used to assess statistical differences (p<0.05).
Results: The highest bacterial attachment was found at worn enamel surfaces. Among the materials, Z and C showed the lowest bacterial attachment regardless of wear (p<0.05).
Conclusions: Bacterial adherence was material dependent and worn surfaces increased bacterial adherence.
IADR/AADR/CADR General Session
2015 IADR/AADR/CADR General Session (Boston, Massachusetts)
Boston, Massachusetts
2015
2150
Dental Materials 5: Biocompatibility and Biologic Effects
  • Ghanta, Haritha  ( University of Pennsylvania , Philadelphia , Pennsylvania , United States )
  • Ozer, Fusun  ( University of Pennsylvania , Philadelphia , Pennsylvania , United States )
  • Yi, Yuanfu  ( The General Hospital of Chinese People's Armed Police Forces , Beijing , China )
  • Pande, Rashmi  ( University of Pennsylvania , Philadelphia , Pennsylvania , United States )
  • Irmak, Ozgur  ( University of Pennsylvania , Philadelphia , Pennsylvania , United States )
  • Dirienzo, Joseph  ( University of Pennsylvania , Philadelphia , Pennsylvania , United States )
  • Blatz, Markus  ( University of Pennsylvania , Philadelphia , Pennsylvania , United States )
  • NONE
    Poster Session
    Prosthodontics and Orthodontics-Antimicrobials
    Friday, 03/13/2015 , 02:00PM - 03:15PM
    Bacterial Attachment
    Bacterial attachment
    Surface Z C A LD E
    Worn 41.40±10.95Aa 56.35±15.48Aa 112.50±23.1Ab 134.75±32.02Abd 158.92±3.06Acd
    Unworn 26.35±8.01Aa 43.65±19.69Aa 88.92±23.42Ab 101.13±29.55Ab 127.25±32.18Abc
    Uppercase letters compare means in each column; lower case letters compare means in each row. Means sharing a same letter are not significantly different.