IADR Abstract Archives
Effect of Material Surfaces on Bacterial Adherence
Objectives: This study compared Streptococcus gordonii adherence to saliva-coated surfaces of enamel (E) and dental materials amalgam (A, Tytin), composite (C, Estelite Sigma Quick), lithium disilicate (LD, IPS e.max), and zirconia (Z, Y-TZP) with and without wear of the surfaces.
Methods: Six sample specimens from each material were prepared and underwent 240,000 cycles of wear with 5 kg (49 N) chewing load with zirconia antagonists in a dual-axis chewing simulator. Specimens were sterilized under UV light and then immersed in filtered saliva collected from one donor. After washing with Phosphate-Buffered Saline (PBS), specimens were placed in individual wells containing 1 x 108 cells/ml of S. gordonii in PBS. Immersed specimens were incubated at 30° C for 2h and washed twice again with PBS, left in Karnovsky’s fixative for 24h, dehydrated, coated with gold-palladium, and examined under SEM. Four images (x 5000) of each sample were taken from worn and unworn surfaces and numbers of the bacteria were counted on the images. Mean values were calculated. ANOVA and post hoc Tukey’s test were used to assess statistical differences (p<0.05).
Results: The highest bacterial attachment was found at worn enamel surfaces. Among the materials, Z and C showed the lowest bacterial attachment regardless of wear (p<0.05).
Conclusions: Bacterial adherence was material dependent and worn surfaces increased bacterial adherence.
Methods: Six sample specimens from each material were prepared and underwent 240,000 cycles of wear with 5 kg (49 N) chewing load with zirconia antagonists in a dual-axis chewing simulator. Specimens were sterilized under UV light and then immersed in filtered saliva collected from one donor. After washing with Phosphate-Buffered Saline (PBS), specimens were placed in individual wells containing 1 x 108 cells/ml of S. gordonii in PBS. Immersed specimens were incubated at 30° C for 2h and washed twice again with PBS, left in Karnovsky’s fixative for 24h, dehydrated, coated with gold-palladium, and examined under SEM. Four images (x 5000) of each sample were taken from worn and unworn surfaces and numbers of the bacteria were counted on the images. Mean values were calculated. ANOVA and post hoc Tukey’s test were used to assess statistical differences (p<0.05).
Results: The highest bacterial attachment was found at worn enamel surfaces. Among the materials, Z and C showed the lowest bacterial attachment regardless of wear (p<0.05).
Conclusions: Bacterial adherence was material dependent and worn surfaces increased bacterial adherence.