IADR Abstract Archives

Effect of Intensive Fluoride Varnish Treatment on Oral Microbiota of Children with ECC

Objectives: Previous studies reported that professionally-applied fluoride varnish (FV) could reduce children’s risk for early childhood caries (ECC), especially in high risk children. The objective of our study was to determine the effect of an intensive FV treatment on Streptococcus mutans colonization and oral microbial composition.
Methods: Forty children (24 boys and 16 girls, mean age (month) = 32.1±10.2) diagnosed with ECC were included in the study. They were given three FV treatments (Durashield varnish, 5% NaF) within three weeks and returned for one- and three-month follow-up visits with additional FV treatments. There were no other modifications in children’s typical diet, oral hygiene or fluoride exposure during the treatment. Pooled supra-gingival plaque samples were collected at baseline and after each treatment. S. mutans levels were evaluated using Dentocult® SM Strip Mutans method. The overall bacterial composition was examined based on PCR-amplified 16S rRNA gene profiles on denaturing gradient gel electrophoresis (DGGE). A subset of bacterial DNA samples was analyzed using the Human Oral Microbe Identification Next Generation Sequencing (HOMINGS) method.
Results: Compared with the baseline, there was a significant reduction in S. mutans levels after the intensive FV treatment at each time point. The reduction remained despite up to a 2-month interval between treatments. DGGE profiles found alterations of overall oral microbial composition, varying from 5% to 65% at the second week after the treatment. HOMINGS data analysis revealed that at genus level a decreased abundance for Streptococcus, Actinobaculum, Alloprevotella, Rothia, Veillonella, and Haemophilus; meanwhile, an increased abundance for Leptotrichia, Neisseria, Selenomonas, and Capnocytophaga after the treatment.
Conclusions: Our preliminary findings suggest that intensive FV treatment can not only reduce S. mutans levels but also influence several commensal bacteria as well as opportunistic pathogens in the oral cavity of children with ECC.
Division: IADR/AADR/CADR General Session
Meeting: 2015 IADR/AADR/CADR General Session (Boston, Massachusetts)
Location: Boston, Massachusetts
Year: 2015
Final Presentation ID: 0228
Abstract Category|Abstract Category(s): Cariology Research - Microbiological Studies / Biofilm
Authors
  • Li, Yihong  ( New York University - , New York , New York , United States )
  • Herman, Neal  ( New York University - , New York , New York , United States )
  • Wang, Zhemeng  ( New York University - , New York , New York , United States )
  • So, Patrick  ( New York University - , New York , New York , United States )
  • Chen, Zhou  ( New York University - , New York , New York , United States )
  • Cotton, Sean  ( The Forsyth Institute , Cambridge , Massachusetts , United States )
  • Kokaras, Alexis  ( The Forsyth Institute , Cambridge , Massachusetts , United States )
  • Paster, Bruce  ( The Forsyth Institute , Cambridge , Massachusetts , United States )
  • Moursi, Amr  ( New York University - , New York , New York , United States )
  • Support Funding Agency/Grant Number: Supported by NYU Student/Faculty Research Funds.
    Financial Interest Disclosure: NONE.
    SESSION INFORMATION
    Oral Session
    Impact of Oral Health Therapies on Oral Biofilms
    Wednesday, 03/11/2015 , 03:15PM - 04:45PM