IADR Abstract Archives
Antifungal Activity of Lichochalcone-A on Candida Albicans Biofilms In Vitro
Objectives: Candida albicans is a major fungal pathogen afflicting a range of disease from superficial mucosal diseases to deep seated mycoses. In the United States, candidiasis is considered the third leading cause of hospital-acquired infection, with reported mortality rate of 40-60%. Biofilm formation is a major virulence factor in pathogenicity of C. albicans, which is believed to cause a high antifungal resistance.
The aim of the present in vitro study was to investigate the influence of lichochalcone-A treatment on the formation and killing of C. albicans biofilms.
Methods: C. albicans (ATCC: 90028) biofilms (n=9) were cultured (2.5 x 10ᶾ CFU/ml) in yeast nitrogen base medium, were grown in sterile 48-wells plate for 48 h at 37oC, 5% CO2 and were treated twice daily with lichochalcone-A (62.5 µM) for 5 days. Fluconazole and solutions with 1% ethanol (v/v) served as positive and vehicle controls, respectively. Biofilms were analyzed for biomass, fungal viability, and polysaccharide composition.
Results: Application of lichochalcone-A reduced the biomass and the polysaccharides composition of C. albicans biofilms compared to those of the vehicle control. In addition, lichochalcone A showed reduction in fungal viability as shown by the decrease in the colony formation unit (CFU).
Conclusions: Lichochalcone-A showed antifungal activity against Candida albicans biofilms in vitro. Consequently, such natural compound may show promise to translate its application into clinical settings.
The aim of the present in vitro study was to investigate the influence of lichochalcone-A treatment on the formation and killing of C. albicans biofilms.
Methods: C. albicans (ATCC: 90028) biofilms (n=9) were cultured (2.5 x 10ᶾ CFU/ml) in yeast nitrogen base medium, were grown in sterile 48-wells plate for 48 h at 37oC, 5% CO2 and were treated twice daily with lichochalcone-A (62.5 µM) for 5 days. Fluconazole and solutions with 1% ethanol (v/v) served as positive and vehicle controls, respectively. Biofilms were analyzed for biomass, fungal viability, and polysaccharide composition.
Results: Application of lichochalcone-A reduced the biomass and the polysaccharides composition of C. albicans biofilms compared to those of the vehicle control. In addition, lichochalcone A showed reduction in fungal viability as shown by the decrease in the colony formation unit (CFU).
Conclusions: Lichochalcone-A showed antifungal activity against Candida albicans biofilms in vitro. Consequently, such natural compound may show promise to translate its application into clinical settings.