IADR Abstract Archives
Systemic Inflammation and the Impact of Obesity on Gingival Inflammation
Objectives: Background: C-Reactive Protein (CRP) is an acute-phase response marker used to measure inflammatory status. Elevated levels of CRP have been detected in the gingival crevicular fluid (GCF) and may be related to either oral or systemic inflammation. In this study, GCF concentrations of CRP, IL-1, IL-8, IL-10, and TNF-α in obese versus normal subjects were obtained to determine whether a correlation exists between CRP levels in serum versus GCF samples. In addition, we investigated the association of Single Nucleotide Polymorphisms (SNPs) in CRP genes and CRP levels in GCF.
Methods: Methods: Recruitment consisted of 15 normocentric and 15 obese subjects of European descent. Anthropometric and clinical measurements (BMI, waist circumference, vitals, blood glucose, blood samples) and a periodontal exam (GCF, gingival index, plaque index, and probing depths) were performed. Concentrations of CRP and cytokines were determined using commercial multiplexed fluorescent bead-based immunoassays. We selected 6 SNPs reported to be genome-wide significant from a previous CRP GWAS study in European Americans. Genotyping was completed using taqman probes. Nonparametric statistical methods and exact tests were emphasized.
Results: Results: GCF CRP levels differed significantly between obese versus non-obese subjects (p=0.0096). GCF CRP correlated moderately with BMI (r=0.46; p=0.01) and waist circumference (r=0.49; p=0.0062). Departure from Hardy-Weinberg proportions was noted only for SNP rs4129267 (p=0.019). We observed a significant association between GCF CRP level and genotype for two of the SNPs (rs4129267, p=0.034 and rs4420638, p=0.032).
Conclusions: Conclusion: Obesity related inflammation influences the physiologic processes of the oral cavity. Further research is warranted to determine the potential for genetic variation to influence oral inflammatory markers. Understanding the dynamics of inflammatory markers in the oral cavity will provide insight for better understanding of the pathogenesis of chronic inflammatory conditions and to facilitate development of diagnostic markers for such conditions.
Supported by NIH CTSA U54TR001013
Methods: Methods: Recruitment consisted of 15 normocentric and 15 obese subjects of European descent. Anthropometric and clinical measurements (BMI, waist circumference, vitals, blood glucose, blood samples) and a periodontal exam (GCF, gingival index, plaque index, and probing depths) were performed. Concentrations of CRP and cytokines were determined using commercial multiplexed fluorescent bead-based immunoassays. We selected 6 SNPs reported to be genome-wide significant from a previous CRP GWAS study in European Americans. Genotyping was completed using taqman probes. Nonparametric statistical methods and exact tests were emphasized.
Results: Results: GCF CRP levels differed significantly between obese versus non-obese subjects (p=0.0096). GCF CRP correlated moderately with BMI (r=0.46; p=0.01) and waist circumference (r=0.49; p=0.0062). Departure from Hardy-Weinberg proportions was noted only for SNP rs4129267 (p=0.019). We observed a significant association between GCF CRP level and genotype for two of the SNPs (rs4129267, p=0.034 and rs4420638, p=0.032).
Conclusions: Conclusion: Obesity related inflammation influences the physiologic processes of the oral cavity. Further research is warranted to determine the potential for genetic variation to influence oral inflammatory markers. Understanding the dynamics of inflammatory markers in the oral cavity will provide insight for better understanding of the pathogenesis of chronic inflammatory conditions and to facilitate development of diagnostic markers for such conditions.
Supported by NIH CTSA U54TR001013