IADR Abstract Archives
Evaluation of Biphasic Calcium Phosphate-based Cement in Rat Carvarial Model
Objectives: A calcium phosphate cement consisting of an α-tricalcium phosphate-tetracalcium phosphate biphasic calcium phosphate (BCP) as the only solid phase was reported (Hirayama et al., this meeting). The BCP based cement provided good handling properties and desired hardening time, and it set to sufficiently high strengths for clinical use. The objective of this study was to evaluate BCP cement when used for repairing artificially created bone defects (∅4mm) in calvaria of rats.
Methods: The test sample was prepared by mixing BCP (Ca/P ratio=1.8) powder with the cement liquid at P/L of 3.0. Commercially available calcium phosphate cements (CPCs), Biopex-R and Osteovation, were used as controls. Surgically created bone defects were filled with the BCP or control cements. At 0, 3 and 8 weeks after surgery, x-ray micro-CT for each surgical site was taken. At 8 weeks after the surgery, the animals were sacrificed, and biopsies were obtained. Subsequently, the sections were subjected to HE and TB staining and examined under a microscope. The value of new bone formation in each surgical site was evaluated quantitatively using NIH image analysis. Student’s t-test was used to establish statistical significance.
Results: The BCP cement paste showed good adaptability to defect sites and exhibited excellent washout resistance. BCP and control cements were gradually resorbed by osteoclast and was replaced by natural bone, but BCP cement consistently showed faster (p<0.05) bone replacement than the control CPCs. These results were also confirmed by x-ray micro-CT observations.
Conclusions: The results indicated that the BCP-based cement has high osteoconductive potential and may be useful for bone defect repairs. Supported by Medical U＆A Inc and Nihon University.
Methods: The test sample was prepared by mixing BCP (Ca/P ratio=1.8) powder with the cement liquid at P/L of 3.0. Commercially available calcium phosphate cements (CPCs), Biopex-R and Osteovation, were used as controls. Surgically created bone defects were filled with the BCP or control cements. At 0, 3 and 8 weeks after surgery, x-ray micro-CT for each surgical site was taken. At 8 weeks after the surgery, the animals were sacrificed, and biopsies were obtained. Subsequently, the sections were subjected to HE and TB staining and examined under a microscope. The value of new bone formation in each surgical site was evaluated quantitatively using NIH image analysis. Student’s t-test was used to establish statistical significance.
Results: The BCP cement paste showed good adaptability to defect sites and exhibited excellent washout resistance. BCP and control cements were gradually resorbed by osteoclast and was replaced by natural bone, but BCP cement consistently showed faster (p<0.05) bone replacement than the control CPCs. These results were also confirmed by x-ray micro-CT observations.
Conclusions: The results indicated that the BCP-based cement has high osteoconductive potential and may be useful for bone defect repairs. Supported by Medical U＆A Inc and Nihon University.