IADR Abstract Archives
Influence of Different Irrigating Solutions Associated to Photodynamic Therapy: In Vivo Study
Objectives: The aim of this study was to evaluate the influence of photodynamic therapy (PDT) and irrigating solutions in dogs' teeth with apical periodontitis.
Methods: Ninety-six roots of premolars, canines and incisors teeth of adult Beagles dogs were used. The teeth were submitted to access opening and pulp extirpation to left the root canals exposed to the oral environment to induce periapical lesion. After 6 months, the root canals were biomechanically prepared 1mm short the apex up to a Protaper F2 and abundantly irrigated. Groups: G1-2.5% sodium hypochlorite + PDT; G2-5% sodium hypochlorite + PDT; G3-2% chlorhexidine + PDT; G4-saline solution + PDT; G5-2.5% sodium hypochlorite; G6-5% sodium hypochlorite; G7-2% chlorhexidine; G8-saline solution; G9-positive control (open tooth); G10-negative control (closed tooth). The root canals that received PDT treatment were filled with curcumin PS 500 mg/L for 5 minutes and activated (Led 450 nm) for 4 minutes with optical fiber 2mm short the apex. The root canals were dried and filled with gutta-percha points and Sealapex by the lateral condensation technique. Six month after treatment, the animals were killed by anesthetic overdose. The specimens were removed, and histologically processed for hematoxylin and eosin and the Brown and Brenn staining. The data were submitted to statistical analysis using the Kruskal-Wallis and Dunn’s multiple comparison tests (p<0,05).
Results: The groups that received PDT treatment demonstrated persistence of inflammation and partial periapical tissue repair that was not significantly different from the groups without PDT (p> 0.05). However, it was significant difference between all treated groups compared to positive and negative controls (p <0.05).
Conclusions: The biomechanical preparation with or without PDT in one session deal with the infection but it did not provide complete repair of periapical tissues.
Methods: Ninety-six roots of premolars, canines and incisors teeth of adult Beagles dogs were used. The teeth were submitted to access opening and pulp extirpation to left the root canals exposed to the oral environment to induce periapical lesion. After 6 months, the root canals were biomechanically prepared 1mm short the apex up to a Protaper F2 and abundantly irrigated. Groups: G1-2.5% sodium hypochlorite + PDT; G2-5% sodium hypochlorite + PDT; G3-2% chlorhexidine + PDT; G4-saline solution + PDT; G5-2.5% sodium hypochlorite; G6-5% sodium hypochlorite; G7-2% chlorhexidine; G8-saline solution; G9-positive control (open tooth); G10-negative control (closed tooth). The root canals that received PDT treatment were filled with curcumin PS 500 mg/L for 5 minutes and activated (Led 450 nm) for 4 minutes with optical fiber 2mm short the apex. The root canals were dried and filled with gutta-percha points and Sealapex by the lateral condensation technique. Six month after treatment, the animals were killed by anesthetic overdose. The specimens were removed, and histologically processed for hematoxylin and eosin and the Brown and Brenn staining. The data were submitted to statistical analysis using the Kruskal-Wallis and Dunn’s multiple comparison tests (p<0,05).
Results: The groups that received PDT treatment demonstrated persistence of inflammation and partial periapical tissue repair that was not significantly different from the groups without PDT (p> 0.05). However, it was significant difference between all treated groups compared to positive and negative controls (p <0.05).
Conclusions: The biomechanical preparation with or without PDT in one session deal with the infection but it did not provide complete repair of periapical tissues.