IADR Abstract Archives
Effect of Recaldent®-Paste with Fluoride on Enamel Remineralization and Demineralization
Objectives: The aim of this study was to investigate and evaluate the effect on remineralization and Demineralization demineralization effect of by a preventives remineralization treatment paste, MI Paste Plus (MIPP, GC) containing Casein Phosphopeptide-Amormous Calcium Phosphate (Recaldent (CPP-ACP, Recaldent®) and fluoride.
Methods: The materials examined in this study were MIPP and Clinpro Tooth Crème (CTC, 3M ESPE). Sound enamel slabs (10×5×1mm) from bovine maxillary incisors were used as a specimens (n=16) for remineralization and demineralization tests. The enamel surfaces of these slabs were polished with SIC paper (#1000). Half of samples were demineralized by 0.1M Lactic acid buffer gel (pH5.0) treatment at 37℃ for 4 days to form the artificial early caries. The re/demineralization effect was evaluated by pH-cycling test for 7 days at 37℃. The demineralization step was achieved by 17hours treatment in 100mM lactic acid solution (pH5.0) and the remineralization step was conducted by immersing samples in 20mM HEPES-OH (pH7.0). After each steps, the specimens were immersed into 10% dilutions of each product for 5 minutes as daily topical treatment. After 7days of pH-cycling, the de/remineralization level was assessed by radiographic image analysis using X-ray CT (TOSCANER-30000, TOSHIBA) and image analysis software (Image J). The areas (vol%・μm) between before and after pH-cycling tested tooth profiles (⊿Z) were calculated. Distilled water (DW) was used as control. Data were statistically analyzed with ANOVA and Turkey test (p<=0.01).
Results: Mean values (standard deviation) of de/remineralization rate are shown below. Demineralization rate of MIPP were significantly lower and remineralization rate were significantly higher than CTC. DW means distilled water as a control.
Conclusions: MIPP shows showed low demineralization rate (inhibition of demineralization) and high remineralization rate. This result indicates MIPP is may be effectiveness as a caries preventive paste.
Methods: The materials examined in this study were MIPP and Clinpro Tooth Crème (CTC, 3M ESPE). Sound enamel slabs (10×5×1mm) from bovine maxillary incisors were used as a specimens (n=16) for remineralization and demineralization tests. The enamel surfaces of these slabs were polished with SIC paper (#1000). Half of samples were demineralized by 0.1M Lactic acid buffer gel (pH5.0) treatment at 37℃ for 4 days to form the artificial early caries. The re/demineralization effect was evaluated by pH-cycling test for 7 days at 37℃. The demineralization step was achieved by 17hours treatment in 100mM lactic acid solution (pH5.0) and the remineralization step was conducted by immersing samples in 20mM HEPES-OH (pH7.0). After each steps, the specimens were immersed into 10% dilutions of each product for 5 minutes as daily topical treatment. After 7days of pH-cycling, the de/remineralization level was assessed by radiographic image analysis using X-ray CT (TOSCANER-30000, TOSHIBA) and image analysis software (Image J). The areas (vol%・μm) between before and after pH-cycling tested tooth profiles (⊿Z) were calculated. Distilled water (DW) was used as control. Data were statistically analyzed with ANOVA and Turkey test (p<=0.01).
Results: Mean values (standard deviation) of de/remineralization rate are shown below. Demineralization rate of MIPP were significantly lower and remineralization rate were significantly higher than CTC. DW means distilled water as a control.
Conclusions: MIPP shows showed low demineralization rate (inhibition of demineralization) and high remineralization rate. This result indicates MIPP is may be effectiveness as a caries preventive paste.