IADR Abstract Archives
DSP and DMP1 as Salivary Biomarkers for Root Resorption
Objectives: Root resorption (RR) is a common complication during comprehensive orthodontic treatment. Currently, RR is diagnostically evaluated via radiographic imaging. Recent studies have shown increased levels of specific biomarkers found in gingival crevicular fluid of patients undergoing orthodontic treatment displaying RR relative to control. The goal of this study is to detect the levels of two dentin matrix proteins in saliva during orthodontic treatment namely, dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1). During tissue remodeling, DMP1 and DSP are released at detectable levels in the saliva of orthodontic patients.
Methods: Patients starting comprehensive orthodontic treatment were recruited to provide saliva samples at 0, 2, 4, and 6 months from the start of treatment. Saliva samples were collected in 50ml centrifuge tubes, vortexed, and centrifuged with protease inhibitor. The samples were then analyzed using enzyme-linked immunosorbent assay (ELISA) to detect our target proteins. Once standard curves were established, a standard ELISA protocol was used to detect DSP and DMP1 using corresponding antibodies. Panoramic radiographs were taken at 0 and 6 months to analyze and diagnose patients undergoing RR.
Results: N=20 saliva samples have been analyzed from 12 study subjects at various stages of the study. DSP and DMP1 were detected in the pre-treatment saliva samples tested with a mean concentration of 61.26pg/mL and 51.87ng/mL respectively. At 2-month measurements the respective concentrations increased to 80.23pg/mL and 148.25ng/mL.
Conclusions: DSP and DMP1 can be readily detected in saliva samples. The goal of this study is to develop biomarkers for root resorption during orthodontic treatment and the amount of proteins in the saliva would be indicative of varying levels of RR. Development of biomarkers would help in developing a non-invasive chair-side test to screen orthodontic patients undergoing RR.
Methods: Patients starting comprehensive orthodontic treatment were recruited to provide saliva samples at 0, 2, 4, and 6 months from the start of treatment. Saliva samples were collected in 50ml centrifuge tubes, vortexed, and centrifuged with protease inhibitor. The samples were then analyzed using enzyme-linked immunosorbent assay (ELISA) to detect our target proteins. Once standard curves were established, a standard ELISA protocol was used to detect DSP and DMP1 using corresponding antibodies. Panoramic radiographs were taken at 0 and 6 months to analyze and diagnose patients undergoing RR.
Results: N=20 saliva samples have been analyzed from 12 study subjects at various stages of the study. DSP and DMP1 were detected in the pre-treatment saliva samples tested with a mean concentration of 61.26pg/mL and 51.87ng/mL respectively. At 2-month measurements the respective concentrations increased to 80.23pg/mL and 148.25ng/mL.
Conclusions: DSP and DMP1 can be readily detected in saliva samples. The goal of this study is to develop biomarkers for root resorption during orthodontic treatment and the amount of proteins in the saliva would be indicative of varying levels of RR. Development of biomarkers would help in developing a non-invasive chair-side test to screen orthodontic patients undergoing RR.