IADR Abstract Archives
Effect of Shockwave Therapy on Orthodontic Tooth Movement
Objectives: Accelerated orthodontic tooth movement has been an area of interest for orthodontic research due to problems associated with longer treatment time. Extracorporeal shockwave therapy (ESWT) is a non-invasive approach using acoustic wave with high-pressure amplitude, small pulse width, and short rise time. It has been shown to induce alveolar bone regeneration, neovascularization, and stem cell differentiation. Due to these features, we speculated that ESWT should be able to accelerate tooth movement. Objectives: we investigated the effect of a single application of ESWT on tooth movement in a rat model.
Methods: In this study, we performed a set of experiments to elucidate the effect of tooth movement in 48 male adult Sprague-Dawley rats. Under general anesthesia, a 10-cN spring was ligated from one first maxillary molar to incisors for 28 days to generate mesial molar movement while the other molar was left intact. A single application of ESWT at a parameter of 1000 pulses at 5 Hz was performed on the day of tooth movement in the ESWT treated group. Faxitron radiograph and microCT were used to measure tooth movement. Histomorphometric analyses were performed to investigate osteoclast activity and bone remodeling. Double fluorescence bone labeling was performed to investigate osteoblast activity. Serum from the rats at day 3, 7 and 28 was subject to ELISA to determine the level of serum RANKL and OPG.
Results: A single application of ESWT at the beginning of tooth movement significantly delayed tooth movement in rats at day 28. Histological studies showed significant increase of osteoclast numbers and increased activity of osteoblasts. The ratio of serum RANKL/OPG showed no difference between groups.
Conclusions: A single application of ESWT delayed tooth movement in rats and interfered physiological balance of osteoblast and osteoclast activities. In addition, ESWT did not affect systemic osteoclast activity.
Methods: In this study, we performed a set of experiments to elucidate the effect of tooth movement in 48 male adult Sprague-Dawley rats. Under general anesthesia, a 10-cN spring was ligated from one first maxillary molar to incisors for 28 days to generate mesial molar movement while the other molar was left intact. A single application of ESWT at a parameter of 1000 pulses at 5 Hz was performed on the day of tooth movement in the ESWT treated group. Faxitron radiograph and microCT were used to measure tooth movement. Histomorphometric analyses were performed to investigate osteoclast activity and bone remodeling. Double fluorescence bone labeling was performed to investigate osteoblast activity. Serum from the rats at day 3, 7 and 28 was subject to ELISA to determine the level of serum RANKL and OPG.
Results: A single application of ESWT at the beginning of tooth movement significantly delayed tooth movement in rats at day 28. Histological studies showed significant increase of osteoclast numbers and increased activity of osteoblasts. The ratio of serum RANKL/OPG showed no difference between groups.
Conclusions: A single application of ESWT delayed tooth movement in rats and interfered physiological balance of osteoblast and osteoclast activities. In addition, ESWT did not affect systemic osteoclast activity.