Clinical Trial About Experimental Solution on Denture Biofilm Using DNA-Checkerboard

Objectives: This cross-over randomized clinical trial investigated the efficacy of an experimental solution (Ricinus communis) for cleaning dentures, comparing it to sodium hypochlorite in different concentrations on the microbial composition of denture biofilm.
Methods: 64 upper denture users (40 healthy and 24 with chronic atrophic candidiasis) were instructed to brush their prosthesis for 3 minutes, three times a day and to immerse them for 20 minutes in the solutions: S1-Saline (control group); S2/S3-0.25 and 0.5% Sodium Hypochlorite; S4-10% Ricinus communis. They were used in a randomized sequence and crossed form for 07 days. A washout period of 7 days was established among the experimental period. After each intervention, biofilms samples were evaluated for the detection of Candida and bacterial species. The microbiological analysis was performed by the DNA-Checkerboard hybridization technique. For the evaluation the solutions on the decrease of total microorganisms count (Pool) was used generalized linear model (GLM; p<0.05). The decrease of each microorganisms count after use of the solutions was evaluated by Friedman Test (p<0.05). The statistical analyzes were realized with aid of software SPSS (SPSS Inc., Chicago, IL, USA).
Results: It was indicated no difference among the groups with and without stomatitis (p=0.748) or interaction between inflammation and solution (p=0.979). There was difference among the solutions (p=0.041). The 10% Ricinus communis (488.72) was similar with the Saline (790.32) and 0.5 and 0.25% sodium hypochlorite (461.45 and 365.93, respectively) which in turn was the most effective and showed equivalence between themselves. 43 different microorganisms were found in the wearer’s denture biofilm and only C. albicans, S. sobrinus and S. aureus showed no significant change in the count depending on the solutions.
Conclusions: It was concluded that there is viability to use Ricinus communis and Sodium Hypochlorite in lower concentrations to the microorganisms control from the complete dentures biofilm.