Osseointegration of Dental Implants

Objectives: The aim of this study was to compare growth of osteoblasts on three different implant surfaces; titanium surface, sandblasted titanium surface, and calcium phosphate coated titanium surface.
Methods: After scientific examination of surface characteristics by way of a Field-Emission Scanning Electron Microscope, MC3T3 osteoblasts were grown on three types of titanium implants: titanium, sandblasted, and calcium phosphate coated. The implants were harvested at 4hr, 8hr, 12hr, 24hr, and 48hr time points. Implants were fixed and stained with rhodamine phalloidin for F-actin expression. Implants were then fixed and prepared on slides with Vector Vectashield mounting medium with Dapi. Slides were examined with Zeiss 200ZM Axiovert fluorescence microscope. All experiments were repeated three times.
Results: There was no difference found in cell number at each time point for each of the three-implant surfaces. There was also no difference found in actin expression between each of the three different implant types at each of the time points.
Conclusions: The quantitative evaluation of cell growth and actin expression on different implant surfaces showed no correlation to time or to surface treatment.