IADR Abstract Archives
SHED Functionally Stimulated by Lipoteichoic Acid in Short Term
Objectives: The aim of this study was to evaluate the behavior of Stem cells from Human Exfoliated Deciduous teeth (SHED) exposed to lipoteichoic acid (LTA), analysing alkaline phosphatase activity, calcium deposition, gene expression and cytokines production.
Methods: SHED cells were treated with LTA, in different concentrations, without and with mineralization induction (MI). Alkaline phosphatase activity was performed to indicate mineralization process. Also, calcium deposition was observed qualitatively by alizarin red staining. Gene expression of DSPP (Dentin Sialophosphoprotein) and DMP-1 (Dentin Matrix Protein-1) was evaluated by PCR. Cytokines IL-1ß (Interleukin-1ß), IL-6 (Interleukin-6), TNF-α (Tumor Necrosis Factor-α) were detected and quantified by ELISA. Statistical analysis of quantitative tests were performed by ANOVA and Tukey test (p<0,05).
Results: In groups without MI, there was an increase in alkaline phosphatase activity of cells exposed to LTA compared to control. Groups with mineralization induction showed more mineral nodules, compared to the groups without MI. After 3 days, cells treated with LTA showed more areas of mineralization compared to control, and after periods of 7 and 14 days were similar. All groups tested expressed genes DSPP and DMP-1, being slightly higher in groups with MI. The expression of IL-1ß and TNF-α by cells of the tested groups were similar, and IL-6 concentration was expressive, with differences among groups and time periods.
Conclusions: According to the methodology and analysis employed in this study, the conclusion is that SHED cells did not suffer interference of bacterial LTA in the expression of IL-1ß, IL-6, TNF-α, and odontoblast differentiation. However, in short term, LTA stimulated cells functionally, with higher deposition of mineral nodules and alkaline phosphatase activity.
Methods: SHED cells were treated with LTA, in different concentrations, without and with mineralization induction (MI). Alkaline phosphatase activity was performed to indicate mineralization process. Also, calcium deposition was observed qualitatively by alizarin red staining. Gene expression of DSPP (Dentin Sialophosphoprotein) and DMP-1 (Dentin Matrix Protein-1) was evaluated by PCR. Cytokines IL-1ß (Interleukin-1ß), IL-6 (Interleukin-6), TNF-α (Tumor Necrosis Factor-α) were detected and quantified by ELISA. Statistical analysis of quantitative tests were performed by ANOVA and Tukey test (p<0,05).
Results: In groups without MI, there was an increase in alkaline phosphatase activity of cells exposed to LTA compared to control. Groups with mineralization induction showed more mineral nodules, compared to the groups without MI. After 3 days, cells treated with LTA showed more areas of mineralization compared to control, and after periods of 7 and 14 days were similar. All groups tested expressed genes DSPP and DMP-1, being slightly higher in groups with MI. The expression of IL-1ß and TNF-α by cells of the tested groups were similar, and IL-6 concentration was expressive, with differences among groups and time periods.
Conclusions: According to the methodology and analysis employed in this study, the conclusion is that SHED cells did not suffer interference of bacterial LTA in the expression of IL-1ß, IL-6, TNF-α, and odontoblast differentiation. However, in short term, LTA stimulated cells functionally, with higher deposition of mineral nodules and alkaline phosphatase activity.