IADR Abstract Archives
Effects of Extracorporeal Shockwave Therapy: A Pilot Study Using a Rat Model
Objectives: This pilot study was designed to assess the effects of extracorporeal shockwave therapy (ECSWT) on dental pulp, brain, lung and osteonecrosis of the jaw in rats.
Methods: Using 400 gram Sprague-Dawley rats osteonecrosis of the jaw (ONJ) was induced in four rats after intraperitoneal injection of 0.1 mg/kg zolendronate thrice weekly for 8 weeks. At the end of the seventh week three adjacent maxillary molars were extracted from the four zolendronate treated rats to induce ONJ, as well as, from an untreated rat (control). Three of the zolendronate treated rats were given extracorporeal shockwave therapy (ECSWT) using a Sanuwave shockwave device post-extraction; one rat received ECSWT (1000 pulses, 240 pulses/minute) over the ONJ area once weekly at weeks 9, 10 &11, two rats received ECSWT over the ONJ area at weeks 9&10. At week 12, a separate control rat was treated with ECSWT over lung, brain and dental pulp areas. All rats were then sacrificed, specimen areas dissected and submitted for microscopic pathologic analysis.
Results: 1. Upon macroscopic examination, the lung tissue demonstrated significant hemorrhage, while all other areas appeared normal.
2. Microscopically, dental pulp tissue appeared normal.
3. Macroscopically the brain tissue appeared normal with no evidence of intracranial hemorrhage.
4. Minimal evidence of ONJ was observed microscopically. The only necrotic bone found was seen in a specimen from a rat that received Zolendroic acid, but was not treated with Sanuwave ECSWT. In the Sanuwave ECSWT-treated groups, no necrotic bone was evidenced; however, significant osseous healing was noted with a profusion of woven bone.
Conclusions: 1. The small sample size precludes strong conclusions.
2. Extracorporeal shockwave therapy appears safe for oral use with no adverse effects on oral or pulpal tissues and may have a positive effect on osseous healing.
3. ECSWT of lung tissues appears contraindicated
Methods: Using 400 gram Sprague-Dawley rats osteonecrosis of the jaw (ONJ) was induced in four rats after intraperitoneal injection of 0.1 mg/kg zolendronate thrice weekly for 8 weeks. At the end of the seventh week three adjacent maxillary molars were extracted from the four zolendronate treated rats to induce ONJ, as well as, from an untreated rat (control). Three of the zolendronate treated rats were given extracorporeal shockwave therapy (ECSWT) using a Sanuwave shockwave device post-extraction; one rat received ECSWT (1000 pulses, 240 pulses/minute) over the ONJ area once weekly at weeks 9, 10 &11, two rats received ECSWT over the ONJ area at weeks 9&10. At week 12, a separate control rat was treated with ECSWT over lung, brain and dental pulp areas. All rats were then sacrificed, specimen areas dissected and submitted for microscopic pathologic analysis.
Results: 1. Upon macroscopic examination, the lung tissue demonstrated significant hemorrhage, while all other areas appeared normal.
2. Microscopically, dental pulp tissue appeared normal.
3. Macroscopically the brain tissue appeared normal with no evidence of intracranial hemorrhage.
4. Minimal evidence of ONJ was observed microscopically. The only necrotic bone found was seen in a specimen from a rat that received Zolendroic acid, but was not treated with Sanuwave ECSWT. In the Sanuwave ECSWT-treated groups, no necrotic bone was evidenced; however, significant osseous healing was noted with a profusion of woven bone.
Conclusions: 1. The small sample size precludes strong conclusions.
2. Extracorporeal shockwave therapy appears safe for oral use with no adverse effects on oral or pulpal tissues and may have a positive effect on osseous healing.
3. ECSWT of lung tissues appears contraindicated
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