IADR Abstract Archives
The effect of acetyl group removal on acemannan's biological activities
Objectives: To identify the role of acetyl group of acemannan, a polysaccharide extracted from Aloe vera gel, on cytotoxicity, cell proliferation and VEGF expression in primary gingival fibroblast
Methods: Deacetylated acemannan (DeAcAM) was prepared using basic hydrolysis and characterized using spectroscopy and chromatography techniques. Primary human gingival fibroblast were treated with DeAcAM. The MTT assay was used for cytotoxicity and cell proliferation. The condition media were collected and measured for VEGF synthesis by commercial ELISA kit. All data were collected and presented as mean ± standard error. The bioactivity assessments were analyzed by one way analysis of variance (ANOVA) followed by post-hoc analysis with Duncan’s Multiple Range Test. Significance was assumed at a p level of < 0.05.
Results: The 10, 35, 50 and 100 percents of deacetylation of acemannan was obtained by incubating with 0.05, 0.1, 0.2, and 0.4 M NaOH, respectively, at 80ο C for 5 minutes. Compared to the serum−free control, all the DeAcAM (4 mg/ml) were not toxic to the human gingival fibroblast. Acemannan and 10% DeAcAM significantly stimulated cell viability compared with the serum-free control group (p<0.05), while the high percentage of deacetylation (≥ 35%) did not. Deacetylation of acemannan results in a significant loss of its ability to promote VEGF expression compared with acemannan (p<0.05). No significant difference was observed between DeAcAM and the serum−free control (p>0.05).
Conclusions: Acetyl groups play an important role for acemannan’s biological activities.
Methods: Deacetylated acemannan (DeAcAM) was prepared using basic hydrolysis and characterized using spectroscopy and chromatography techniques. Primary human gingival fibroblast were treated with DeAcAM. The MTT assay was used for cytotoxicity and cell proliferation. The condition media were collected and measured for VEGF synthesis by commercial ELISA kit. All data were collected and presented as mean ± standard error. The bioactivity assessments were analyzed by one way analysis of variance (ANOVA) followed by post-hoc analysis with Duncan’s Multiple Range Test. Significance was assumed at a p level of < 0.05.
Results: The 10, 35, 50 and 100 percents of deacetylation of acemannan was obtained by incubating with 0.05, 0.1, 0.2, and 0.4 M NaOH, respectively, at 80ο C for 5 minutes. Compared to the serum−free control, all the DeAcAM (4 mg/ml) were not toxic to the human gingival fibroblast. Acemannan and 10% DeAcAM significantly stimulated cell viability compared with the serum-free control group (p<0.05), while the high percentage of deacetylation (≥ 35%) did not. Deacetylation of acemannan results in a significant loss of its ability to promote VEGF expression compared with acemannan (p<0.05). No significant difference was observed between DeAcAM and the serum−free control (p>0.05).
Conclusions: Acetyl groups play an important role for acemannan’s biological activities.