Adsorption Effect of Heat-treated Hydrotalcite Materials on Hydrogen Sulfide by Bacteria Adsorption Effect of Heat-treated Hydrotalcite Materials on Hydrogen Sulfide by Bacteria

Objectives: We attempt to develop the microporous material (hydrotalsite) that absorb volatile sulfur compounds (VSC), such as hydrogen sulfide (H₂S). Hydrotalsite (HT) was synthesized and their adsorption property of H₂S has been studied. Moreover, we have shown the amount of H₂S production for Fusobacterium nucleatum (F. nucleatum) was higher than other periodontopathic bacteria using gas chromatography (GC). This study evaluated the adsorption effects of heat-treated HT materials on production of H₂S induced by F. nucleatum.
Methods: F. nucleatum was inoculated in Brain Heart Infusion broth with and without adding HT or heated HT at 500℃ for 30 minutes (HT-500) or heated HT at 800℃ for 30 minutes (HT-800) in a tightly sealed container at 37℃ under anaerobic condition. Gas produced by each culture was collected by aspiration using a gas tight syringe. H₂S concentrations were measured by GC. Numerical data were statistically analyzed using one–way ANOVA and Scheffe’s multiple comparison test (P<0.05).
Results: H₂S concentrations (mean±SD, ppm) were1221.4±349.1, 781.3±131.4, 242.3±70.1 and 245.1±18.3 in the without HT, HT, HT-500, and HT-800, respectively. HT-500 and HT-800 to the culture significantly decreased in the amount of H₂S produced by F. nucleatum.
Conclusions: These results suggest that the HT-500 is expected to be likely absorbent for removal of VSC from the mouth.