IADR Abstract Archives
Association between S. sanguinis and caries-free oral environment in the initial and mature plaques
Objectives: To quantify S. mutans and S. sanguinis in initial and mature plaque between 2 groups and to analyze the association between caries-related factors and these bacteria in each group.
Methods: Supra gingival plaque were obtained from 138 Thai children aged 2-6 years old (S-ECC=68, CF=72). Plaque and gingival indices were recorded. Firstly, an overnight mature plaque was obtained then after a thoroughly prophylaxis, initial plaque was collected 4 hrs. after. Assessed parent attitude and behavior in child’s oral hygiene care and diet using questionnaire. After DNA extraction, quantitative real-time PCR using SYBR green was performed.
Results: For initial plaque, S. sanguinis was higher in CF. S. mutans (0.011) and
S. mutans/S. sanguinis ratio (0.005, Mann-Whitney U test at p<0.05) were higher in S-ECC.
S. sanguinis amount was inverse correlated with dmft (0.00), gingival index (0.044), and plaque index (0.011, Spearman correlation test at p<0.05). For mature plaque, S. mutans (0.00) and S. mutans/S. sanguinis ratio (0.005, Mann-Whitney U test at p<0.05) were also higher in S-ECC. There was a positive correlation between S. mutans, S. mutans/S. sanguinis ratio and dmft (0.00, Spearman correlation test at p<0.05). There were different in parent education levels (0.041) and bottle sleeping or breast feeding habit (0.019, Chi square test at p<0.05) between 2 groups. From multivariate analysis, child who has S. sanguinis in initial plaque <105 (cfu/ml) is 5.27 chance of child who has S. sanguinis>105 in develop S-ECC, child who came from low family income (<10,000 baht/month) is 2.885 chance in develop S-ECC of child who came from family income >10,000 and child who was bottle sleeping is 2.601 chance in develop S-ECC of child who was not.
Conclusions: The amount of S. sanguinis in the initial plaque, low family income and bottle sleeping are important factors in determining risk of S-ECC.
Methods: Supra gingival plaque were obtained from 138 Thai children aged 2-6 years old (S-ECC=68, CF=72). Plaque and gingival indices were recorded. Firstly, an overnight mature plaque was obtained then after a thoroughly prophylaxis, initial plaque was collected 4 hrs. after. Assessed parent attitude and behavior in child’s oral hygiene care and diet using questionnaire. After DNA extraction, quantitative real-time PCR using SYBR green was performed.
Results: For initial plaque, S. sanguinis was higher in CF. S. mutans (0.011) and
S. mutans/S. sanguinis ratio (0.005, Mann-Whitney U test at p<0.05) were higher in S-ECC.
S. sanguinis amount was inverse correlated with dmft (0.00), gingival index (0.044), and plaque index (0.011, Spearman correlation test at p<0.05). For mature plaque, S. mutans (0.00) and S. mutans/S. sanguinis ratio (0.005, Mann-Whitney U test at p<0.05) were also higher in S-ECC. There was a positive correlation between S. mutans, S. mutans/S. sanguinis ratio and dmft (0.00, Spearman correlation test at p<0.05). There were different in parent education levels (0.041) and bottle sleeping or breast feeding habit (0.019, Chi square test at p<0.05) between 2 groups. From multivariate analysis, child who has S. sanguinis in initial plaque <105 (cfu/ml) is 5.27 chance of child who has S. sanguinis>105 in develop S-ECC, child who came from low family income (<10,000 baht/month) is 2.885 chance in develop S-ECC of child who came from family income >10,000 and child who was bottle sleeping is 2.601 chance in develop S-ECC of child who was not.
Conclusions: The amount of S. sanguinis in the initial plaque, low family income and bottle sleeping are important factors in determining risk of S-ECC.