IADR Abstract Archives

Effect of DDC on dentin MMPs

Objective: The study aimed to use zymography and in-situ zymography techniques to evaluate the ability of dicyclohexylcarbodiimide (DDC), a potent cross-linker to inhibit dentin matrix metalloproteinases (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) 0.5M DDC pre-treatment prevents this enzymatic activity.

Methods:  Zymography and in-situ zymography were performed on protein extracts obtained from dentin powder treated with Scotchbond 1XT (SB1XT) with or without 0.5M DDC pre-treatment. In addition adhesive/dentin interfaces were bonded with SB1XT applied on acid-etched dentin slabs pre-treated or not with 0.5M DDC conditioner and submitted to in situ zymography analysis.

 

Results : Zymograms revealed increased expression of dentin endogenous MMPs after SB1XT application, while the use of DDC primer inhibited dentin gelatinases. Results of in situ zymography revealed that hybrid layers of SB1XT bonded specimens exposed to fluorescein-conjugated gelatine exhibited intense collagenolytic activity, while reduced fluorescence signal at the adhesive/dentin interface was found in specimens pre-treated with 0.5M DCC. Control sections incubated with standard non-fluorescent gelatine showed no fluorescence.

Conclusion : DCC effectively inhibits dentin MMPs when used as primer on acid-etched dentin. This occurs through crosslinking of carboxylic acids to primary amines, such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). However, DCC has the advantage to be soluble in acetone or ethanol and might be blended within adhesive formulations. Future studies are needed to evaluate the efficacy of experimental DCC primer in dentin bonding procedures.

IADR/AMER General Session
2014 IADR/AMER General Session (Cape Town, South Africa)
Cape Town, South Africa
2014
31
Dental Materials 1: Adhesion - Bond Strength Testing and Mechanisms
  • Breschi, Lorenzo  ( DIBINEM University of Bologna, Bologna, , Italy )
  • Angeloni, Valeria  ( University of Trieste, Trieste, N/A, Italy )
  • Fontanive, Luca  ( University of Trieste, Trieste, , Italy )
  • Scotti, Nicola  ( University of Turin, Turin, N/A, Italy )
  • Cadenaro, Milena  ( University of Trieste, Trieste, , Italy )
  • Tay, Franklin  ( Georgia Regents University, Augusta, GA, USA )
  • Pashley, David  ( Georgia Regents University, Augusta, GA, USA )
  • Mazzoni, Annalisa  ( University of Trieste, Trieste, , Italy )
    • Oral Session
    Bond Durability; Effects of Treatments and Solutions on Collagen, Enzymes and Others
    06/25/2014