IADR Abstract Archives

Role of PDGF and  TGF-β in T-PRF induced Bone Formation  

Objective: In our previous studies, we developed a novel platelet-rich product that we called titanium-prepared platelet-rich fibrin (T-PRF). T-PRF is based on the hypothesis that titanium may be more effective at activating platelets than the silica activators used with glass tubes in Chouckroun’s platelet-rich fibrin (PRF). Our first results showed that T-PRF membrane can induce the formation of new bone in a rabbit calvaria model. This study aimed to assess the effects of platelet-derived growth factor (PDGF) and transforming growth factor-beta (TGF-β) on T-PRF induced bone formation. 

Method: Twenty-four adult male New Zealand rabbits were used in the study. T-PRF alone, anorganic bovine bone (ABB), and T-PRF + ABB were used in the experimental groups. No materials were used in the control group. Half of the animals were sacrificed after 1 month, and the remaining animals were sacrificed after 3 months. New hard and soft healing tissue was examined by immunocytochemistry to localise PDGF, and TGF-β positive cells.

Result: T-PRF membrane remains were observed in the rabbit tissues after 1 month.  More TGF-β positive cells were noted in the T-PRF only group than in the control, ABB, and T-PRF + ABB groups after 1 month. 

Conclusion: Based on the results of this study, T-PRF membrane was able to remain in the rabbit tissues for at least 1 month, which was sufficient time for the initiation of formation of new bone. TGF-β could be an important growth factor in accelerating the early bone healing events of T-PRF bone augmentation in a rabbit bone-healing model.

IADR/AMER General Session
2014 IADR/AMER General Session (Cape Town, South Africa)
Cape Town, South Africa
2014
118
Periodontal Research - Therapy
  • Tunali, Mustafa  ( GATA Haydarpasa, Istanbul, N/A, Turkey )
  • Özdemir, Hakan  ( Cummufiyet Universitesi, Sivas, N/A, Turkey )
  • Küçükodaci, Zafer  ( GATA Haydarpasa, Istanbul, N/A, Turkey )
  • Ezirganli, Seref  ( Assist. Prof., Istanbul, , Turkey )
  • Baris, Emre  ( Assist. Prof., Ankara, , Turkey )
  • Akman, Serhan  ( Selcuk University, Konya, N/A, Turkey )
  • Firatli, Erhan  ( Istanbul University, Faculty of Dentistry, Istanbul, N/A, Turkey )
    • Oral Session
    Keynote Address; Advances in Periodontal Regeneration
    06/25/2014