Cytoprotective Effects of Opuntia Ficus-Indica Extract
Objective: Searching for new biological activity of nopal extract. We investigated whether water and ethanol nopal extract shows cytoprotective activity against viral infection, ultraviolet (UV) irradiation and chemotherapeutic drug treatment used in dentistry targeted to oral squamous cell carcinoma (OSCC).
Method: The water and ethanol extract of Opuntia ficus-indica (WEO and EEO, respectively) were prepared. The viability of the mock-infected, HIV-infected, UV-irradiated, drug-treated cells without or with the extract was determined by MTT method. Radical scavenging activity was determined colorimetrically and by ESR spectroscopy. Caspase-3 activity in the cell lysate was measured by substrate cleavage assay.
Result: Both extracts did not show anti-HIV activity, but protected the cells from cell injury induced by UV-irradiation, possibly due to the slight inhibition of caspase-3 activation. WEO dose-dependently scavenged the superoxide anion, hydroxyl and DPPH radicals. WEO also significantly inhibited cytotoxicity of methotrexate, doxorubicin and 5-FU.
Conclusion: The cytoprotective activity of nopal extracts against UV and anti-tumor agents may be partly due to its radical scavenging activity.
Division: IADR/AMER General Session
Meeting:2014 IADR/AMER General Session (Cape Town, South Africa) Location: Cape Town, South Africa
Year: 2014 Final Presentation ID:1634 Abstract Category|Abstract Category(s):Pharmacology/Therapeutics/Toxicology
Authors
Mena Acra, Alejandro
( Universidad Autónoma del Estado de México, Toluca, Estado de México, , Mexico
; Meikai University School of Dentistry, Sakado, , Japan
)
Nakashima, Hideki
( St. Marianna University School of Medicine, Kawasaki, , Japan
)
Visoso-salgado, Ángel
( Universidad Autónoma del Estado de México, Toluca, Estado de México, , Mexico
)
Montiel Bastida, Norma
( Universidad Autónoma del Estado de México, Toluca, Estado de México, , Mexico
)
Sakagami, Hiroshi
( Meikai University School of Dentistry, Sakado, , Japan
)
Matsuta, Tomohiko
( Okinawa Industrial Technology Center, Okinawa, , Japan
)
Adachi, Kazunori
( Meikai University School of Dentistry, Sakado, , Japan
)
Otsuki, Sumiko
( Meikai University School of Dentistry, Sakado, , Japan
)
Nakajima, Hiroshi
( Meikai University School of Dentistry, Sakado, , Japan
)
Satoh, Kazue
( School of Medicine, Showa University, Tokyo, , Japan
)
Kanamoto, Taisei
( St. Marianna University School of Medicine, Kawasaki, , Japan
)
Terakubo, Shigemi
( St. Marianna University School of Medicine, Kawasaki, , Japan
)