Method: The records of the patients with discomfort in the mouth who visited the department of oral medicine, Seoul National Dental Hospital were reviewed. The samples were taken from the oral mucosa and tongue with sterile cottons. The cotton was rolled over the surface of DentocultRCA and the tubes were incubated for two days. The other cotton was inserted in the transport media (TransystemTM) and delivered to the microbial laboratory. The detection rates for two methods were examined by McNemar test. Candidaspecies cultured in SDA were identified by a fully automated VITEK 2 system.
Result: The cultures were performed from 328 subjects (age 62.3+/- 13.6 years) consisted of 248 females and 80 males. The numbers of both positive and negative for two cultures were 124 and 157, respectively. Dentocult®CA(+) and SDA(-) were 27, and Dentocult®CA(-) and SDA(+) were 20. The detection rates between two methods were not statistically different. C. albicans (111 out of 144 samples, 77.1%) was the most frequently identified, and C. glabrata(20, 13.9%), C. tropicalis(3, 2.1%) were followed.
Conclusion: C. albicans was the most frequently identified in SDA culture from the oral swab. Dentocult®CA compared with SDA is ready to use and has longer expire period. This method can be used for OC in the general dental clinic without special equipments and no needs to deliver the swab samples to the laboratory.