IADR Abstract Archives
Effect of 660nm LED on Wound Healing in Fibroblast Cell
Method: A photo illumination-cell culture system was designed to evaluate the wound healing of fibroblast like cells exposed to 600 nm LED light. The HGF-1 cells were placed on 12-well plates, and exposed to 660 nm red LED light with an intensity of 2.5, 5.5 and 8.5 mW/cm2 for exposure times of 5, 10, and 20 min. The in vitrowound healing determined by irradiation with the red LED light was measured at 5, 10 and 20 min after exposure. In each plate, artificial wounds, approximately 0.4–0.5 mm in width, were scratched into the monolayer using a sterile plastic 10 μl micropipette tip.
Result: Scratch wounds were made to determine the effects of red LED light irradiation on HGF-1 cell migration. As shown in Table, the rate of HGF-1 cell migration was increased after 5, 10 and 20 min irradiation compared to that of the control at 12 h after wounding (*p<0.05).
| ExposureTime | |||||
| Period | 0 min | 5 min | 10 min | 20 min | |
| Cell migration rate (μm/hr) | 12 h | 2.08±0.24 | 2.92±0.32* | 3.17±0.42* | 2.75±0.21* |
| 24 h | 2.08±0.34 | 2.08±0.16 | 2.50±0.23* | 2.29±0.15 |
Conclusion: The optimal intensity of 660 nm red LEDs to stimulate and promte cell migration in the 5 and 10 min exposure groups of HGF-1 cells was 8.5 mW/cm2. These results suggest that 660 nm red LED might be a good source for phototherapy in wound healing medical treatments.