IADR Abstract Archives

Photodynamic Antimicrobial Therapy of Curcumin Against Cariogenic Pathogens

Objective: The present study evaluated the susceptibility of planktonic cultures of S. mutans (ATCC 25175) and L. acidophilus(ATCC-IAL-523) from the Adolfo Lutz Institute (IAL) to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. 

Method: Bacterial suspensions of S. mutans and L. acidophilus isolated (as single species) and combined (multspecies) were prepared and then evaluated. Four different groups were analyzed: L-D- (control group), L-D+ (drug group), L+D- (light group), and L+D+ (photodynamic therapy group). Two different concentrations of curcumin were tested (0.75 and 1.5 g/L) associated with a 5.7 J/cm2light emission diode. 

Result: Significant decreases (p < 0.05) in the viability of S. mutans were only observed when the bacterial suspensions were exposed to both curcumin and light. Then, reductions in viability of up to 99.99% were observed when using 1.5 g/L of the photosensitizer. The susceptibility of L. acidophilus was considerably lower (21% and 37.6%) for both curcumin concentrations. Conclusion: , Photodynamic therapy was found to be effective in reducing S. mutans and L. acidophiluson planktonic cultures. 

Conclusion: No significant reduction was found for L-D+, proving the absence of dark toxicity of the drug.

Research Support Foundation of São Paulo State (FAPESP), Studies and Projects Financier (FINEP), and Science and Technology Support Foundation of Pernambuco State (FACEPE).

Division: IADR/AADR/CADR General Session
Meeting: 2013 IADR/AADR/CADR General Session (Seattle, Washington)
Location: Seattle, Washington
Year: 2013
Final Presentation ID: 1811
Abstract Category|Abstract Category(s): Late-breaking News
Authors
  • Carneiro, Vanda  ( UNIVERSIDADE DE PERNAMBUCO, Camaragibe, N/A, Brazil )
  • Araújo, Natália  ( Universidade de Pernambuco, Camaragibe, , Brazil )
  • Fontana, Carla  ( Universidade Estadual Paulista, Araraquara, SP, N/A, Brazil )
  • Bagnato, Vanderlei  ( IFSC-University of São Paulo, São Carlos, N/A, Brazil )
  • Gerbi, Marleny  ( UNIVERSIDADE DE PERNAMBUCO, Camaragibe, N/A, Brazil )
  • SESSION INFORMATION
    Poster Session
    Late-breaking News II
    03/22/2013