IADR Abstract Archives

Effect of fluoride-releasing dental materials on artificial bacteria-induced secondary caries

Objective: To study the effect of experimental antibacterial fluoride-releasing dental materials (composite and bonding agent) to inhibit secondary caries using an in vitro artificial bacteria-induced caries model. Method: Class 5 cavities (3 mm high, 3-4 mm wide, 2-3 mm deep, n=8) were prepared near enamel-cementum junction on the buccal and palatal surfaces of each tooth. The prepared teeth were restored with three groups of materials: Group 1: Experimental antibacterial fluoride-releasing bonding agent and composite, Group 2: commercial fluoride-releasing materials (Xeno IV bonding agent and Esthet•X composites, Caulk/Dentsply), Group 3: commercial non-fluoride-releasing materials (Adper™ Scotchbond™ MP bonding agent and Filtek Supreme Plus composites, 3M-ESPE). The composites were applied and light-cured (40s) layer-by-layer or following manufacturers' instructions. After restoration, the specimens were painted with nail polish with 1.5 mm of enamel exposed around the restoration margin, incubated in Brain Heart Infusion broth containing 1% sucrose and inoculated with Streptococcus mutans UA159 and Lactobacillus casei at 37°C for 8 days, with the medium changed every two days. Then the specimens were removed from the culture medium, cleaned, air dried, imbedded in epoxy resin, sectioned and polished to less than 0.25 mm, photographed under a Nikon i50 polarized light. The wall lesions depth from the enamel surface was measured at 25 μm distance from the adhesive-enamel interface. The data were analyzed using ANOVA and post hoc Tukey test (a=0.05).

Result: Table 1. Wall lesion depth of artificial caries (mean±SD).

Materials	Wall lesion depth (μm)
Group 1: Experimental antibacterial fluoride-releasing materials	137.14±29.06^A
Group 2: commercial fluoride-releasing materials.	168.00±31.12^B
Group3: commercial non-fluoride-releasing materials	235.64±19.60^C

The groups with different superscript letters have significant difference (p<0.05).

Conclusion: The experimental antibacterial fluoride-releasing dental materials have significantly higher inhibition effect to secondary caries than commercial F-releasing or non-F-releasing dental materials. (Supported by NIH/NIDCR grant R01DE019203).

Division: IADR/AADR/CADR General Session
Meeting: 2013 IADR/AADR/CADR General Session (Seattle, Washington)
Location: Seattle, Washington
Year: 2013
Final Presentation ID: 621
Abstract Category|Abstract Category(s): Dental Materials 5: Biocompatibility and Biologic Effects

Authors

Zhang, Jian-feng ( Louisiana State University, New Orleans, LA, USA )

Costin, Stephen A. ( Louisiana State University, New Orleans, LA, USA )

Xu, Xiaoming ( Louisiana State University, New Orleans, LA, USA )

Wang, Yapin ( Louisiana State University, New Orleans, LA, USA )

Lacour, Letitie A. ( Louisiana State University, New Orleans, LA, USA )

Townsend, Janice A. ( Louisiana State University, New Orleans, LA, USA )

Fan, Yuwei ( Louisiana State University, New Orleans, LA, USA )

Liao, Sumei ( Louisiana State University, New Orleans, LA, USA )

Wen, Zezhang T. ( Louisiana State University, New Orleans, LA, USA )

Davis, Matthew B. ( Louisiana State University, New Orleans, LA, USA )

Li, Shibao ( Louisiana State University, New Orleans, LA, USA )

SESSION INFORMATION

Poster Session
Biofilm and Anticaries
03/21/2013