IADR Abstract Archives

Antibacterial Effect of Carbodiimide (EDC) Against Caries-Related Microorganisms

Objective: Ethyldimethylamino propyl carbodiimide (EDC) is a cross-linker currently being investigated as a potential inhibitor of dentin collagen degradation. However, there is no information in the literature concerning its antimicrobial effect against oral microorganisms. Thus, the aim of this study was to investigate the antimicrobial activity of EDC against caries-related bacteria and Candida albicans.

Method: Standard strains of S. mutans (ATCC 25175), L. acidophilus (ATCC 4356), S. sobrinus (ATCC 27607) and Candida albicans (ATCC 90028) were cultivated and submitted to agar diffusion test. Seven solutions were tested: Sorensen's buffer (control), 0.1M, 0.3M, 0.5M, 1M and 2M EDC, 0.12% chlorhexidine (CHX) or nystatin. Filter paper disks (n=6/solution) were impregnated with 5 μL of each solution and placed in Petri dishes containing the specific culture medium. After 48h of incubation, the inhibition zones were measured. For L. acidophilus, the minimum bactericidal concentration (MBC) and the antibacterial effect in monospecies biofilm (XTT test) were also investigated. Data were submitted to ANOVA, Tukey and Mann-Whitney (α=0.05).

Result: For the agar diffusion test, EDC exhibited inhibitory effect only against L. acidophilus at concentrations equal to or higher than 0.5M, with no statistical difference between 0.5, 1 and 2M. However, irrespective of the concentration, that effect was inferior than the effect seen for 0.12% CHX. The MBC for both planktonic and biofilm growth was defined as 0.03M.

Conclusion: Low concentrations of EDC are capable of inhibiting the growth of L. acidophilus even when in monospecies biofilm. This study was partially supported by FAPESP 2012/08866-4 and R01 DE015306 NIH/NIDCR.

IADR/AADR/CADR General Session
2013 IADR/AADR/CADR General Session (Seattle, Washington)
Seattle, Washington
2013
620
Dental Materials 5: Biocompatibility and Biologic Effects
  • Estrêla, Renata  ( Universidade Est. Paulista Julio Mesquita, Goiânia, Goiás, N/A, Brazil )
  • Francisconi, Renata  ( Universidade Est. Paulista Julio Mesquita, Araraquara, N/A, Brazil )
  • Scheffel, Débora L.  ( UNESP - Univ. Estadual Paulista, Araraquara, Sao Paulo, N/A, Brazil )
  • Nogueira, Marianne Nicole  ( Universidade Est. Paulista Julio Mesquita, São Paulo, N/A, Brazil )
  • Spolidorio, Denise Palomari  ( UNESP - Univ Estadual Paulista, Araraquara, N/A, Brazil )
  • Costa, Carlos  ( Universidade Est. Paulista Julio Mesquita, Araraquara, São Paulo, N/A, Brazil )
  • Pashley, David  ( Georgia Health Sciences University, Augusta, GA, USA )
  • Hebling, Josimeri  ( Araraquara Dental School - UNESP, Araraquara - SP, , Brazil )
    • Poster Session
    Biofilm and Anticaries
    03/21/2013