Effects of Novel TheraCal Formulation Extracts on OD21 Cells

Effects of TheraCal Material Extraction on Cell OD21

Yantong Wang, Satin Salehi, John C. Mitchell, Byoung In Suh

Objectives: The purpose of this study was to evaluate the effect of three novel pulp capping compounds on the growth of immortalized murine pulp cells (OD-21).

Methods: Light-cured discs (D=10mm x 1mm) of TheraCal LC (LC), TheraCal DC (Experimental mixture DC) and TheraBag65 LC (Experimental mixture TB3) (N=4 each) were soaked in Dulbecco's Modified Eagle Medium (DMEM) for 4 days at 37°C. The discs were removed and the extraction solution was filtered and supplemented with 10% Fetal Bovine Serum. OD-21 cells were placed into 24-well culture plates at 10Kcells/well and allowed to grow for 24 hours in control media at 37°C 5% CO₂. Wells (N=5each) were treated once (24 hours), or twice (24 and 48 hours) with extract media and followed over a four day period for the once-treated group, or over three days for the twice-treated group. Cells grown in standard α-MEM with FBS were used as a control. Cell growth/number was assessed by culturing the cells for 1 hour daily in 10% AlamarBlue media and measuring the metabolic product using a fluorescent microplate reader. Percentages of control fluorescence were compared using ANOVA with α=0.05.

Results: The cell growth/number (standard deviations) after one and two treatments are shown in the figures below. Differences were examined by ANOVA with α=0.05.

Conclusions: At every time point, cell survival following the first treatment with all three materials tested, were all similar and greater than 80%. The Day3 cell survival rate following the second treatments showed differences between materials, with DC and TB superior to LC, however by day 4, only DC remained significantly different from the other two materials. All three materials tested were not detrimental to OD21 cells.